| Literature DB >> 6314408 |
M Pomponi, L Spanò, M G Sabbadini, P Costantino.
Abstract
The root-inducing plasmid of the agropine type Agrobacterium rhizogenes 1855 was mapped by means of the restriction endonuclease EcoRI. The circular arrangement of the more than 60 fragments generated by this enzyme was established by electrophoretic analysis of pBR322 clones harboring overlapping segments of pRi1855 derived by partial digestion with EcoRI. A large region of the plasmid comprising the T-DNA was mapped with two additional enzymes, BamHI and HindIII, by means of Southern blot hybridizations between the fragments generated by the three enzymes.Entities:
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Year: 1983 PMID: 6314408 DOI: 10.1016/0147-619x(83)90064-1
Source DB: PubMed Journal: Plasmid ISSN: 0147-619X Impact factor: 3.466