A common organization of the T-DNA genes expressed in plant hairy roots induced by different plasmids of Agrobacterium rhizogenes.

The recently described pathogenic plasmid from Agrobacterium rhizogenes 2659 induces "hairy root" proliferation in infected plants which synthesize a new opine type, provisionally called cucumopine. The T-DNA restriction site map of 2659 differs from other pRi T-DNAs, i.e., mannopine and agropine. However all these three different T-DNAs have a similar organization as detected through hybridization. This, previously shown by our cross-hybridization studies between mannopine and agropine pRi T-DNAs, is here extended to the cucumopine pRi T-DNA; each of the three pRi T-DNA is composed of successive DNA regions that would find actual counterparts in the two other T-DNAs. Equivalence of DNA regions is suggested by their similar length, location and organization in the three T-DNAs and by their sequence homology substantial enough to be reproducibly cross-hybridized in all our experiments. This suggests that the three pRi T-DNAs share most of their functions. This was sought by analysing the pRi 2659 T-DNA gene expression in transformed plant tissues. The mRNA species observed in pRi 2659 carrot hairy roots of in vitro cultures give a pattern that is rather unvariable and resembles those described for some agropine type pRi transformant tissues. This transcript pattern is consistent with the preservation in the pRi 2659 T-DNA of counterparts of most of the open reading frames detected in the TL-DNA of agropine type pRis.