Literature DB >> 6310580

Inducible transcription of five globin genes in K562 human leukemia cells.

A Dean, T J Ley, R K Humphries, M Fordis, A N Schechter.   

Abstract

We studied the abundance and structure of globin mRNAs present in K562 cells both before and after induction of hemoglobin synthesis by hemin. In vitro translation of poly(A)+ RNA from K562 cells generated protein products corresponding to alpha, A gamma-, G gamma-, epsilon-, and zeta-globin mRNAs. Individual globin mRNAs increased 1.5- to 3-fold after induction. Similar results were obtained by measuring steady-state mRNA concentrations of induced and uninduced cells by using S1 nuclease mapping. Globin gene transcripts were correctly initiated and processed. In addition, S1 nuclease analysis revealed the presence of delta-globin mRNA in both control and induced cells. A small percentage of delta-globin transcripts appeared to be initiated upstream from the normal initiation site. beta-Globin mRNA was not detected in any studies. The results (i) suggest that hemin induction of K562 cells is mediated at a transcriptional level and (ii) reveal the dissociation of delta- and beta-globin gene expression in K562 cells compared with normal erythroid cells.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6310580      PMCID： PMC384288          DOI： 10.1073/pnas.80.18.5515

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  25 in total

1.  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.

Authors:  C B Lozzio; B B Lozzio
Journal:  Blood       Date:  1975-03       Impact factor: 22.113

2.  Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.

Authors:  S L Adams; M E Sobel; B H Howard; K Olden; K M Yamada; B de Crombrugghe; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

3.  Spliced early mRNAs of simian virus 40.

Authors:  A J Berk; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

4.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.

Authors:  T R Rutherford; J B Clegg; D J Weatherall
Journal:  Nature       Date:  1979-07-12       Impact factor: 49.962

5.  Human globin gene expression: control of beta, delta and delta beta chain production.

Authors:  W G Wood; J M Old; A V Roberts; J B Clegg; D J Weatherall
Journal:  Cell       Date:  1978-10       Impact factor: 41.582

6.  Electrophoretic separation of human embryonic globin demonstrates "alpha-thalassemia" in human leukemia cell line K562.

Authors:  B P Alter; S C Goff
Journal:  Biochem Biophys Res Commun       Date:  1980-06-16       Impact factor: 3.575

7.  Embryonic-fetal erythroid characteristics of a human leukemic cell line.

Authors:  E J Benz; M J Murnane; B L Tonkonow; B W Berman; E M Mazur; C Cavallesco; T Jenko; E L Snyder; B G Forget; R Hoffman
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205

8.  Transcriptional regulation of globin gene expression in the human erythroid cell line K562.

Authors:  P Charnay; T Maniatis
Journal:  Science       Date:  1983-06-17       Impact factor: 47.728

9.  Abnormal human haemoglobins. Separation and characterization of the alpha and beta chains by chromatography, and the determination of two new variants, hb Chesapeak and hb J (Bangkok).

Authors:  J B Clegg; M A Naughton; D J Weatherball
Journal:  J Mol Biol       Date:  1966-08       Impact factor: 5.469

10.  Disorders of human hemoglobin.

Authors:  A Bank; J G Mears; F Ramirez
Journal:  Science       Date:  1980-02-01       Impact factor: 47.728
View more
  52 in total

1.  Quantitative analysis of globin gene induction in single human erythroleukemic cells.

Authors:  R D Smith; J D Malley; A N Schechter
Journal:  Nucleic Acids Res       Date:  2000-12-15       Impact factor: 16.971

2.  The role of trans-acting factors and DNA-bending in the silencing of human beta-globin gene expression.

Authors:  L R Drew; D C Tang; P E Berg; G P Rodgers
Journal:  Nucleic Acids Res       Date:  2000-07-15       Impact factor: 16.971

3.  Specific repression of beta-globin promoter activity by nuclear ferritin.

Authors:  R H Broyles; V Belegu; C R DeWitt; S N Shah; C A Stewart; Q N Pye; R A Floyd
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-31       Impact factor: 11.205

4.  Inducibility of the HS II enhancer depends on binding of an erythroid specific nuclear protein.

Authors:  P A Ney; B P Sorrentino; C H Lowrey; A W Nienhuis
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

5.  Cooperative activities of hematopoietic regulators recruit RNA polymerase II to a tissue-specific chromatin domain.

Authors:  Kirby D Johnson; Jeffrey A Grass; Meghan E Boyer; Carol M Kiekhaefer; Gerd A Blobel; Mitchell J Weiss; Emery H Bresnick
Journal:  Proc Natl Acad Sci U S A       Date:  2002-08-22       Impact factor: 11.205

6.  Characterization of an unique RNA initiated immediately upstream from human alpha 1 globin gene in vivo and in vitro: polymerase II-dependence, tissue specificity, and subcellular location.

Authors:  J Hess; C Perez-Stable; A Deisseroth; C K Shen
Journal:  Nucleic Acids Res       Date:  1985-09-11       Impact factor: 16.971

7.  Beta-globin gene promoter generates 5' truncated transcripts in the embryonic/fetal erythroid environment.

Authors:  K Khazaie; F Gounari; M Antoniou; E deBoer; F Grosveld
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

8.  Molecular mechanism governing heme signaling in yeast: a higher-order complex mediates heme regulation of the transcriptional activator HAP1.

Authors:  L Zhang; A Hach; C Wang
Journal:  Mol Cell Biol       Date:  1998-07       Impact factor: 4.272

9.  Transcription of the HS2 enhancer toward a cis-linked gene is independent of the orientation, position, and distance of the enhancer relative to the gene.

Authors:  S Kong; D Bohl; C Li; D Tuan
Journal:  Mol Cell Biol       Date:  1997-07       Impact factor: 4.272

10.  Synergism between hypersensitive sites confers long-range gene activation by the beta-globin locus control region.

Authors:  E H Bresnick; L Tze
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-29       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.