Literature DB >> 6310510

Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli.

H Weber, C Weissmann.   

Abstract

We describe a method for the formation of hybrid genes by in vivo recombination between two genes with partial sequence homology. DNA structures consisting of plasmid vector sequences, flanked by the alpha 2 interferon gene on the one side and a portion of the alpha 1 interferon gene (homology about 80%) on the other, were transfected into E. coli SK1592. Appropriate resistance markers allowed the isolation of colonies containing circular plasmids which arose by in vivo recombination between the partly homologous interferon gene sequences. Eleven different recombinant genes were identified, six of which encoded new hybrid interferons not easily accessible by recombinant DNA techniques.

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Year:  1983        PMID： 6310510      PMCID： PMC326304          DOI： 10.1093/nar/11.16.5661

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Isolation of covalently closed circular DNA of high molecular weight from bacteria.

Authors:  T C Currier; E W Nester
Journal:  Anal Biochem       Date:  1976-12       Impact factor: 3.365

2.  A family of structural genes for human lymphoblastoid (leukocyte-type) interferon.

Authors:  G Allen; K H Fantes
Journal:  Nature       Date:  1980-10-02       Impact factor: 49.962

3.  The structure of eight distinct cloned human leukocyte interferon cDNAs.

Authors:  D V Goeddel; D W Leung; T J Dull; M Gross; R M Lawn; R McCandliss; P H Seeburg; A Ullrich; E Yelverton; P W Gray
Journal:  Nature       Date:  1981-03-05       Impact factor: 49.962

4.  Human leukocyte interferon: isolation and characterization of several molecular forms.

Authors:  M Rubinstein; W P Levy; J A Moschera; C Y Lai; R D Hershberg; R T Bartlett; S Pestka
Journal:  Arch Biochem Biophys       Date:  1981-08       Impact factor: 4.013

5.  Nucleotide sequence of the kanamycin resistance transposon Tn903.

Authors:  A Oka; H Sugisaki; M Takanami
Journal:  J Mol Biol       Date:  1981-04-05       Impact factor: 5.469

6.  A subcloning strategy for DNA sequence analysis.

Authors:  A M Frischauf; H Garoff; H Lehrach
Journal:  Nucleic Acids Res       Date:  1980-12-11       Impact factor: 16.971

Review 7.  Structure and expression of human IFN-alpha genes.

Authors:  C Weissmann; S Nagata; W Boll; M Fountoulakis; A Fujisawa; J I Fujisawa; J Haynes; K Henco; N Mantei; H Ragg; C Schein; J Schmid; G Shaw; M Streuli; H Taira; K Todokoro; U Weidle
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1982-09-24       Impact factor: 6.237

8.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

9.  Target cell specificity of two species of human interferon-alpha produced in Escherichia coli and of hybrid molecules derived from them.

Authors:  M Streuli; A Hall; W Boll; W E Stewart; S Nagata; C Weissmann
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

10.  Antiviral activities of hybrids of two major human leukocyte interferons.

Authors:  P K Weck; S Apperson; N Stebbing; P W Gray; D Leung; H M Shepard; D V Goeddel
Journal:  Nucleic Acids Res       Date:  1981-11-25       Impact factor: 16.971
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  17 in total

1.  Construction of a series of ompC-ompF chimeric genes by in vivo homologous recombination in Escherichia coli and characterization of their translational products.

Authors:  T Mizuno; H Kasai; S Mizushima
Journal:  Mol Gen Genet       Date:  1987-05

2.  Construction of a series of ompF-ompC chimeric genes by in vivo homologous recombination in Escherichia coli and characterization of the translational products.

Authors:  T Nogami; T Mizuno; S Mizushima
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490

3.  Simultaneous synthesis of human-, mouse- and chimeric epidermal growth factor genes via 'hybrid gene synthesis' approach.

Authors:  W L Sung; D M Zahab; F L Yao; R Wu; S A Narang
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

Review 4.  Genetic engineering of antiviral agents.

Authors:  A S Huang; J Böni
Journal:  Antimicrob Agents Chemother       Date:  1987-09       Impact factor: 5.191

5.  Intramolecular recombination between partially homologous sequences in Escherichia coli and Xenopus laevis oocytes.

Authors:  J P Abastado; S Darche; F Godeau; B Cami; P Kourilsky
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

6.  Chimeric transcriptional activators generated in vivo from VnfA and AnfA of Azotobacter vinelandii: N-terminal domain of AnfA is responsible for dependence on nitrogenase Fe protein.

Authors:  E Frise; A Green; M Drummond
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

7.  Mechanism of intramolecular recyclization and deletion formation following transformation of Escherichia coli with linearized plasmid DNA.

Authors:  E C Conley; V A Saunders; V Jackson; J R Saunders
Journal:  Nucleic Acids Res       Date:  1986-11-25       Impact factor: 16.971

8.  Mechanism of Streptococcus mutans glucosyltransferases: hybrid-enzyme analysis.

Authors:  Y J Nakano; H K Kuramitsu
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

9.  Repression of the murine interferon alpha 11 gene: identification of negatively acting sequences.

Authors:  A Civas; M Dion; G Vodjdani; J Doly
Journal:  Nucleic Acids Res       Date:  1991-08-25       Impact factor: 16.971

10.  Recombinant human adenoviruses containing hybrid adenovirus type 5 (Ad5)/Ad12 E1A genes: characterization of hybrid E1A proteins and analysis of transforming activity and host range.

Authors:  T Jelinek; F L Graham
Journal:  J Virol       Date:  1992-07       Impact factor: 5.103
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