Literature DB >> 6258146

A subcloning strategy for DNA sequence analysis.

A M Frischauf, H Garoff, H Lehrach.   

Abstract

We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid. Ordered sets of subclones of predetermined overlap are are generated. These can be sequenced directly without further strand- or fragment separation steps.

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Year:  1980        PMID： 6258146      PMCID： PMC324324          DOI： 10.1093/nar/8.23.5541

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

2.  In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.

Authors:  F Heffron; M So; B J McCarthy
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

3.  A strategy of DNA sequencing employing computer programs.

Authors:  R Staden
Journal:  Nucleic Acids Res       Date:  1979-06-11       Impact factor: 16.971

4.  Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.

Authors:  A Panet; J H van de Sande; P C Loewen; H G Khorana; A J Raae; J R Lillehaug; K Kleppe
Journal:  Biochemistry       Date:  1973-12-04       Impact factor: 3.162

5.  A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13.

Authors:  P H Schreier; R Cortese
Journal:  J Mol Biol       Date:  1979-03-25       Impact factor: 5.469

6.  The use of thin acrylamide gels for DNA sequencing.

Authors:  F Sanger; A R Coulson
Journal:  FEBS Lett       Date:  1978-03-01       Impact factor: 4.124

7.  Computer programs for the assembly of DNA sequences.

Authors:  T R Gingeras; J P Milazzo; D Sciaky; R J Roberts
Journal:  Nucleic Acids Res       Date:  1979-09-25       Impact factor: 16.971

8.  A method for sequencing restriction fragments with dideoxynucleoside triphosphates.

Authors:  J Maat; A J Smith
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
  9 in total
  71 in total

1.  Autogenous regulation of the gene for transcription termination factor rho in Escherichia coli: localization and function of its attenuators.

Authors:  Y Matsumoto; K Shigesada; M Hirano; M Imai
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

2.  Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames.

Authors:  N K Menon; J Robbins; H D Peck; C Y Chatelus; E S Choi; A E Przybyla
Journal:  J Bacteriol       Date:  1990-04       Impact factor: 3.490

3.  Ordered deletions for DNA sequencing and in vitro mutagenesis by polymerase extension and exonuclease III gapping of circular templates.

Authors:  S Henikoff
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

4.  DNA sequence of a class I pseudogene from the Tla region of the murine MHC: recombination at a B2 alu repetitive sequence.

Authors:  D A Fisher; M Pecht; L Hood
Journal:  J Mol Evol       Date:  1989-04       Impact factor: 2.395

5.  Anatomy of the herpes simplex virus 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants.

Authors:  P E Pellett; K G Kousoulas; L Pereira; B Roizman
Journal:  J Virol       Date:  1985-01       Impact factor: 5.103

6.  Penicillin acylase from E. coli: unique gene-protein relation.

Authors:  G Schumacher; D Sizmann; H Haug; P Buckel; A Böck
Journal:  Nucleic Acids Res       Date:  1986-07-25       Impact factor: 16.971

7.  Transcription initiation sites and nucleotide sequence of a herpes simplex virus 1 gene conserved in the Epstein-Barr virus genome and reported to affect the transport of viral glycoproteins.

Authors:  P E Pellett; F J Jenkins; M Ackermann; M Sarmiento; B Roizman
Journal:  J Virol       Date:  1986-12       Impact factor: 5.103

8.  Expression and DNA sequence of RED1, a gene required for meiosis I chromosome segregation in yeast.

Authors:  E A Thompson; G S Roeder
Journal:  Mol Gen Genet       Date:  1989-08

9.  Methods to investigate the expression of lignin peroxidase genes by the white rot fungus Phanerochaete chrysosporium.

Authors:  J Reiser; I S Walther; C Fraefel; A Fiechter
Journal:  Appl Environ Microbiol       Date:  1993-09       Impact factor: 4.792

10.  Target cell specificity of two species of human interferon-alpha produced in Escherichia coli and of hybrid molecules derived from them.

Authors:  M Streuli; A Hall; W Boll; W E Stewart; S Nagata; C Weissmann
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205
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