Literature DB >> 6308670

Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1.

S van der Werf, C Wychowski, P Bruneau, B Blondel, R Crainic, F Horodniceanu, M Girard.   

Abstract

Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the beta-lactamase sequence of Escherichia coli plasmid pBR322. Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-beta-lactamase fusion protein that reacted with antibodies raised against poliovirus capsid polypeptide VP1 and with a monoclonal poliovirus type 1 neutralizing antibody, C3. Deletions of various lengths were generated within the VP1 sequence. The hybrid proteins expressed by the deleted plasmids did not react any more with C3 when the region of VP1 amino acids 95-110 (poliovirus nucleotides 2,754-2,806) was deleted. Therefore, the C3 epitope responsible for virus neutralization is most probably located in this region of the capsid polypeptide.

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Year:  1983        PMID： 6308670      PMCID： PMC384192          DOI： 10.1073/pnas.80.16.5080

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  Detection by monoclonal antibodies of an antigenic determinant critical for poliovirus neutralization present on VP1 and on heat-inactivated virions.

Authors:  B Blondel; O Akacem; R Crainic; P Couillin; F Horodniceanu
Journal:  Virology       Date:  1983-04-30       Impact factor: 3.616

2.  Identification of a poliovirus neutralization epitope through use of neutralizing antiserum raised against a purified viral structural protein.

Authors:  E A Emini; A J Dorner; L F Dorner; B A Jameson; E Wimmer
Journal:  Virology       Date:  1983-01-15       Impact factor: 3.616

3.  Location and primary structure of a major antigenic site for poliovirus neutralization.

Authors:  P D Minor; G C Schild; J Bootman; D M Evans; M Ferguson; P Reeve; M Spitz; G Stanway; A J Cann; R Hauptmann; L D Clarke; R C Mountford; J W Almond
Journal:  Nature       Date:  1983-02-24       Impact factor: 49.962

4.  Isolated poliovirus capsid protein VP1 induces a neutralizing response in rats.

Authors:  M Chow; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

5.  [Structural polypeptide VP1 in poliovirus type 1 induced by neutralizing antibodies].

Authors:  B Blondel; R Crainic; F Horodniceanu
Journal:  C R Seances Acad Sci III       Date:  1982-01-11

6.  Expression of poliovirus capsid polypeptide VP1 in Escherichia coli.

Authors:  S van der Werf; M Dréano; P Bruneau; H Kopecka; M Girard
Journal:  Gene       Date:  1983-07       Impact factor: 3.688

7.  Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence.

Authors:  J L Bittle; R A Houghten; H Alexander; T M Shinnick; J G Sutcliffe; R A Lerner; D J Rowlands; F Brown
Journal:  Nature       Date:  1982-07-01       Impact factor: 49.962

8.  Comparison of the amino acid sequence of the major immunogen from three serotypes of foot and mouth disease virus.

Authors:  A J Makoff; C A Paynter; D J Rowlands; J C Boothroyd
Journal:  Nucleic Acids Res       Date:  1982-12-20       Impact factor: 16.971

9.  Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies.

Authors:  E A Emini; B A Jameson; A J Lewis; G R Larsen; E Wimmer
Journal:  J Virol       Date:  1982-09       Impact factor: 5.103

10.  Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.

Authors:  A Nomoto; T Omata; H Toyoda; S Kuge; H Horie; Y Kataoka; Y Genba; Y Nakano; N Imura
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205
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  28 in total

1.  Molecular basis for linkage of a continuous and discontinuous neutralization epitope on the structural polypeptide VP2 of poliovirus type 1.

Authors:  K J Wiegers; K Wetz; R Dernick
Journal:  J Virol       Date:  1990-03       Impact factor: 5.103

2.  Permissive sites and topology of an outer membrane protein with a reporter epitope.

Authors:  A Charbit; J Ronco; V Michel; C Werts; M Hofnung
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

3.  Mutations conferring resistance to neutralization with monoclonal antibodies in type 1 poliovirus can be located outside or inside the antibody-binding site.

Authors:  B Blondel; R Crainic; O Fichot; G Dufraisse; A Candrea; D Diamond; M Girard; F Horaud
Journal:  J Virol       Date:  1986-01       Impact factor: 5.103

4.  Genetic insertion and exposure of a reporter epitope in the ferrichrome-iron receptor of Escherichia coli K-12.

Authors:  G S Moeck; B S Bazzaz; M F Gras; T S Ravi; M J Ratcliffe; J W Coulton
Journal:  J Bacteriol       Date:  1994-07       Impact factor: 3.490

5.  CS31A capsule-like antigen as an exposure vector for heterologous antigenic determinants.

Authors:  F Bousquet; C Martin; J P Girardeau; M C Méchin; M Der Vartanian; H Laude; M Contrepois
Journal:  Infect Immun       Date:  1994-06       Impact factor: 3.441

6.  Structural factors modulate the activity of antigenic poliovirus sequences expressed on hybrid hepatitis B surface antigen particles.

Authors:  F Delpeyroux; E Van Wezel; B Blondel; R Crainic
Journal:  J Virol       Date:  1990-12       Impact factor: 5.103

7.  Cell-induced conformational change in poliovirus: externalization of the amino terminus of VP1 is responsible for liposome binding.

Authors:  C E Fricks; J M Hogle
Journal:  J Virol       Date:  1990-05       Impact factor: 5.103

8.  Regions of poliovirus protein VP1 produced in Escherichia coli induce neutralizing antibodies.

Authors:  M E Hoatlin; O M Kew; M E Renz
Journal:  J Virol       Date:  1987-05       Impact factor: 5.103

9.  Vaccination against poliomyelitis: present possibilities and future prospects.

Authors:  J L Melnick
Journal:  Am J Public Health       Date:  1988-03       Impact factor: 9.308

10.  In vitro phenotypic markers of a poliovirus recombinant constructed from infectious cDNA clones of the neurovirulent Mahoney strain and the attenuated Sabin 1 strain.

Authors:  M Kohara; T Omata; A Kameda; B L Semler; H Itoh; E Wimmer; A Nomoto
Journal:  J Virol       Date:  1985-03       Impact factor: 5.103
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