Literature DB >> 6307277

The interaction of purified rabbit bone collagenase with purified rabbit bone metalloproteinase inhibitor.

T E Cawston, G Murphy, E Mercer, W A Galloway, B L Hazleman, J J Reynolds.   

Abstract

1. Pure rabbit bone metalloproteinase inhibitor (TIMP) bound tightly to pure rabbit bone collagenase with an apparent Kd of 1.4 X 10(-10) M. 2. The molecular weight of the enzyme-inhibitor complex was found to be 54 000, but no enzyme activity could be recovered from the complex after treatment with either mercurials or proteinases. The complex thus differed from latent collagenase in terms of size, susceptibility to mercurials and behaviour on concanavalin A-Sepharose. 3. The interaction of the purified components was compared with that of crude collagenase and crude inhibitor in culture medium. Mercurial treatment partially reversed the inhibition in the crude system, but not when the purified components were used. 4. The significance of the results is discussed in relation to the extracellular control of the activity of collagenase.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6307277      PMCID： PMC1154361          DOI： 10.1042/bj2110313

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  22 in total

1.  A rapid and reproducible assay for collagenase using [1-14C]acetylated collagen.

Authors:  T E Cawston; A J Barrett
Journal:  Anal Biochem       Date:  1979-11-01       Impact factor: 3.365

2.  A specific inhibitor of vertebrate collagenase produced by human skin fibroblasts.

Authors:  H G Welgus; G P Stricklin; A Z Eisen; E A Bauer; R V Cooney; J J Jeffrey
Journal:  J Biol Chem       Date:  1979-03-25       Impact factor: 5.157

3.  Inhibitor of human collagenase from cultures of human tendon.

Authors:  C A Vater; C L Mainardi; E D Harris
Journal:  J Biol Chem       Date:  1979-04-25       Impact factor: 5.157

4.  The detection and characterisation of collagenase inhibitors from rabbit tissues in culture.

Authors:  G Murphy; E C Cartwright; A Sellers; J J Reynolds
Journal:  Biochim Biophys Acta       Date:  1977-08-11

5.  Evidence that latent collagenases are enzyme-inhibitor complexes.

Authors:  A Sellers; E Cartwright; G Murphy; J J Reynolds
Journal:  Biochem J       Date:  1977-05-01       Impact factor: 3.857

6.  A new factor that may control collagen resorption.

Authors:  J J Reynolds; G Murphy; A Sellers; E Cartwright
Journal:  Lancet       Date:  1977-08-13       Impact factor: 79.321

7.  Purification of pig synovial collagenase to high specific activity.

Authors:  T E Cawston; J A Tyler
Journal:  Biochem J       Date:  1979-12-01       Impact factor: 3.857

8.  Human skin collagenase: isolation of precursor and active forms from both fibroblast and organ cultures.

Authors:  G P Stricklin; E A Bauer; J J Jeffrey; A Z Eisen
Journal:  Biochemistry       Date:  1977-04-19       Impact factor: 3.162

9.  Collagenase production by rheumatoid synovial cells: stimulation by a human lymphocyte factor.

Authors:  J M Dayer; R Graham; G Russell; S M Krane
Journal:  Science       Date:  1977-01-14       Impact factor: 47.728

10.  Endogenous activation of latent collagenase by rheumatoid synovial cells. Evidence for a role of plasminogen activator.

Authors:  Z Werb; C L Mainardi; C A Vater; E D Harris
Journal:  N Engl J Med       Date:  1977-05-05       Impact factor: 91.245
View more
  31 in total

1.  Regulation of the expression of tissue inhibitor of metalloproteinases and collagenase by retinoids and glucocorticoids in human fibroblasts.

Authors:  S D Clark; D K Kobayashi; H G Welgus
Journal:  J Clin Invest       Date:  1987-11       Impact factor: 14.808

2.  Rapid purification of tissue inhibitor of metalloproteinases from human plasma and identification as a gamma-serum protein.

Authors:  T E Cawston; D N Noble; G Murphy; A J Smith; C Woodley; B Hazleman
Journal:  Biochem J       Date:  1986-09-15       Impact factor: 3.857

3.  Neutral metalloproteinases produced by human mononuclear phagocytes. Enzyme profile, regulation, and expression during cellular development.

Authors:  H G Welgus; E J Campbell; J D Cury; A Z Eisen; R M Senior; S M Wilhelm; G I Goldberg
Journal:  J Clin Invest       Date:  1990-11       Impact factor: 14.808

4.  Collagen degradation in the pregnant human cervix at term and after prostaglandin-induced cervical ripening.

Authors:  W Rath; B C Adelmann-Grill; U Pieper; W Kuhn
Journal:  Arch Gynecol       Date:  1987

5.  Direct extraction and assay of bone tissue collagenase and its relation to parathyroid-hormone-induced bone resorption.

Authors:  Y Eeckhout; J M Delaissé; G Vaes
Journal:  Biochem J       Date:  1986-11-01       Impact factor: 3.857

6.  Platelet-derived collagenase inhibitor: characterization and subcellular localization.

Authors:  T W Cooper; A Z Eisen; G P Stricklin; H G Welgus
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

7.  Purification of a metalloproteinase inhibitor from human rheumatoid synovial fluid.

Authors:  E Mercer; T E Cawston; M de Silva; B L Hazleman
Journal:  Biochem J       Date:  1985-11-01       Impact factor: 3.857

8.  Identification of tissue inhibitor of metalloproteinase-2 (TIMP-2)-progelatinase complex as the third metalloproteinase inhibitor peak in rheumatoid synovial fluid.

Authors:  T E Cawston; H F Bigg; I M Clark; B L Hazleman
Journal:  Ann Rheum Dis       Date:  1993-03       Impact factor: 19.103

9.  Extracts of human articular cartilage contain an inhibitor of tissue metalloproteinases.

Authors:  D D Dean; J F Woessner
Journal:  Biochem J       Date:  1984-02-15       Impact factor: 3.857

10.  Tissue inhibitor of metalloproteinases and collagenase inhibitory activity in lung secretions from patients with chronic obstructive bronchitis: effect of corticosteroid treatment.

Authors:  D Burnett; J J Reynolds; R V Ward; S C Afford; R A Stockley
Journal:  Thorax       Date:  1986-10       Impact factor: 9.139
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.