Literature DB >> 6304626

Characterization of a genomic clone for rat seminal vesicle secretory protein IV.

J C Kandala, M K Kistler, R P Lawther, W S Kistler.   

Abstract

The entire coding region for rat seminal vesicle secretory protein IV was obtained on a 3.5 kb Eco RI fragment isolated from a genomic library in lambda Charon 4A. The coding sequence for SVS IV message is interrupted twice by introns. The first lies just downstream from the juncture of the 21 amino acid secretory signal peptide with the start of the mature protein, and the second lies in the 3'-nontranslated region. The major transcriptional start site was mapped by primer extention and is 22 nucleotides upstream from the translational initiation codon. S1 protection experiments indicated additional minor transcriptional starts about 27 and 50 nucleotides further upstream from the major cap site. The entire transcriptional unit comprises about 1740 nucleotides. The SVS IV gene does not belong to an obvious gene family, and it is conserved in mice and guinea pigs.

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Year:  1983        PMID： 6304626      PMCID： PMC325956          DOI： 10.1093/nar/11.10.3169

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  57 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

3.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

4.  EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.

Authors:  P Leder; D Tiemeier; L Enquist
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

5.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

6.  A simple method for the preparation of large quantities of pure plasmid DNA.

Authors:  G O Humphreys; G A Willshaw; E S Anderson
Journal:  Biochim Biophys Acta       Date:  1975-04-02

7.  Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Authors:  M W McDonell; M N Simon; F W Studier
Journal:  J Mol Biol       Date:  1977-02-15       Impact factor: 5.469

8.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

9.  The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA.

Authors:  B Hudson; W B Upholt; J Devinny; J Vinograd
Journal:  Proc Natl Acad Sci U S A       Date:  1969-03       Impact factor: 11.205

10.  Nucleotide sequence of the rightward operator of phage lambda.

Authors:  T Maniatis; A Jeffrey; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205
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  12 in total

1.  The N-terminal 1-16 peptide derived in vivo from protein seminal vesicle protein IV modulates alpha-thrombin activity: potential clinical implications.

Authors:  Marilena Lepretti; Susan Costantini; Gaetano Ammirato; Gaia Giuberti; Michele Caraglia; Angelo M Facchiano; Salvatore Metafora; Paola Stiuso
Journal:  Exp Mol Med       Date:  2008-10-31       Impact factor: 8.718

2.  Sequence organisation of rat seminal vesicle F gene: location of transcriptional start point and sequence comparison with six other androgen-regulated genes.

Authors:  L Williams; C McDonald; S Higgins
Journal:  Nucleic Acids Res       Date:  1985-02-11       Impact factor: 16.971

3.  The 11S rat seminal vesicle mRNA directs the in vitro synthesis of two precursors of the major secretory protein IV.

Authors:  S Metafora; J Guardiola; G Paonessa; P Abrescia
Journal:  Nucleic Acids Res       Date:  1984-02-24       Impact factor: 16.971

Review 4.  Compilation of published signal sequences.

Authors:  M E Watson
Journal:  Nucleic Acids Res       Date:  1984-07-11       Impact factor: 16.971

5.  Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.

Authors:  M Kozak
Journal:  Nucleic Acids Res       Date:  1984-01-25       Impact factor: 16.971

6.  Effect of protein SV-IV on experimental Salmonella enterica serovar Typhimurium infection in mice.

Authors:  Caterina Romano-Carratelli; Concetta Bentivoglio; Immacolata Nuzzo; Nunzia Benedetto; Elisabetta Buommino; Anna Cozzolino; Maria Cartenì; Francesco Morelli; Maria Rosaria Costanza; Biancamaria Metafora; Vittoria Metafora; Salvatore Metafora
Journal:  Clin Diagn Lab Immunol       Date:  2002-01

7.  Polymorphism of rat seminal vesicle secretory proteins: characterization of svp-1 and svp-2 and their identification with the major secretory proteins IV and V.

Authors:  A Maffei; G Paonessa; P Abrescia; S Metafora; J Guardiola
Journal:  Biochem Genet       Date:  1984-06       Impact factor: 1.890

8.  Seminal vesicle secretion IV gene: allelic difference due to a series of 20-base-pair direct tandem repeats within an intron.

Authors:  S E Harris; P E Mansson; D B Tully; B Burkhart
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

9.  SV-IV Peptide1-16 reduces coagulant power in normal Factor V and Factor V Leiden.

Authors:  Biagio Di Micco; Marilena Lepretti; Lidia Rota; Ilaria Quaglia; Paola Ferrazzi; Gianluca Di Micco; Pierpaolo Di Micco
Journal:  J Transl Med       Date:  2007-12-21       Impact factor: 5.531

10.  Synthesis of a testosterone-dependent secretory protein by rat seminal vesicle-derived cell lines.

Authors:  G F Tajana; P Locuratolo; S Metafora; P Abrescia; J Guardiola
Journal:  EMBO J       Date:  1984-03       Impact factor: 11.598
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