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Literature DB >> 6300833

Tumor induction by direct injection of cloned v-src DNA into chickens.

Y K Fung, L B Crittenden, A M Fadly, H J Kung.

Abstract

The in vitro transforming potential of Rous sarcoma viruses has been shown to reside in a gene segment, v-src. To provide direct proof that the isolated v-src sequence contains all the information necessary for sarcoma induction in vivo, we have injected subgenomic and genomic cloned DNA carrying v-src directly into chickens. We report that v-src, without the complements of other viral genes, can effectively induce sarcomas in the host within weeks after injection. The sarcoma tumors generated by subgenomic v-src DNA are of mono- or oligoclonal origin. They have acquired v-src sequences in the genome and expressed high levels of src messages. Intact long terminal repeats do not seem to be essential in the tumorigenic process.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1983 PMID： 6300833 PMCID： PMC393375 DOI： 10.1073/pnas.80.2.353

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

27 in total

1. Identification of a transformation-specific antigen induced by an avian sarcoma virus.

Authors: J S Brugge; R L Erikson
Journal: Nature Date: 1977-09-22 Impact factor: 49.962

2. Protein kinase activity associated with the avian sarcoma virus src gene product.

Authors: M S Collett; R L Erikson
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

3. The size and genetic composition of virus-specific RNAs in the cytoplasm of cells producing avian sarcoma-leukosis viruses.

Authors: S R Weiss; H E Varmus; J M Bishop
Journal: Cell Date: 1977-12 Impact factor: 41.582

4. Mechanism of transfection of chicken embryo fibroblasts by Rous sarcoma virus DNA.

Authors: G M Cooper; S Okenquist
Journal: J Virol Date: 1978-10 Impact factor: 5.103

5. Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.

Authors: A D Levinson; H Oppermann; L Levintow; H E Varmus; J M Bishop
Journal: Cell Date: 1978-10 Impact factor: 41.582

6. A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors: F L Graham; A J van der Eb
Journal: Virology Date: 1973-04 Impact factor: 3.616

7. Properties of a normal mouse cell DNA sequence (sarc) homologous to the src sequence of Moloney sarcoma virus.

Authors: M Oskarsson; W L McClements; D G Blair; J V Maizel; G F Vande Woude
Journal: Science Date: 1980-03-14 Impact factor: 47.728

8. Endogenous viral genes are non-essential in the chicken.

Authors: S M Astrin; E G Buss; W S Haywards
Journal: Nature Date: 1979-11-15 Impact factor: 49.962

9. Assays for endogenous and exogenous lymphoid leukosis viruses and chick helper factor with RSV(--) cell lines.

Authors: L B Crittenden; D A Eagen; F A Gulvas
Journal: Infect Immun Date: 1979-05 Impact factor: 3.441

10. Viral DNA in bursal lymphomas induced by avian leukosis viruses.

Authors: P Neiman; L N Payne; R A Weiss
Journal: J Virol Date: 1980-04 Impact factor: 5.103

18 in total

1. Tumor induction by the LTR, v-src, LTR DNA in four B (MHC) congenic lines of chickens.

Authors: J Svoboda; J Plachý; J Hejnar; I Karakoz; R V Guntaka; J Geryk
Journal: Immunogenetics Date: 1992 Impact factor: 2.846

2. Two adaptor proteins differentially modulate the phosphorylation and biophysics of Kv1.3 ion channel by SRC kinase.

Authors: Karen K Cook; Debra A Fadool
Journal: J Biol Chem Date: 2002-01-25 Impact factor: 5.157

3. Immunological responsiveness against tumors induced by avian sarcoma virus: reduced expression of pp60src kinase activity in regressing tumors.

Authors: L Poulin; L Grisé-Miron; M A Wainberg
Journal: J Virol Date: 1985-03 Impact factor: 5.103

Tumor induction by direct injection of cloned v-src DNA into chickens.

1. Identification of a transformation-specific antigen induced by an avian sarcoma virus.

2. Protein kinase activity associated with the avian sarcoma virus src gene product.

3. The size and genetic composition of virus-specific RNAs in the cytoplasm of cells producing avian sarcoma-leukosis viruses.

4. Mechanism of transfection of chicken embryo fibroblasts by Rous sarcoma virus DNA.

5. Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.

6. A new technique for the assay of infectivity of human adenovirus 5 DNA.

7. Properties of a normal mouse cell DNA sequence (sarc) homologous to the src sequence of Moloney sarcoma virus.

8. Endogenous viral genes are non-essential in the chicken.

9. Assays for endogenous and exogenous lymphoid leukosis viruses and chick helper factor with RSV(--) cell lines.

10. Viral DNA in bursal lymphomas induced by avian leukosis viruses.

1. Tumor induction by the LTR, v-src, LTR DNA in four B (MHC) congenic lines of chickens.

2. Two adaptor proteins differentially modulate the phosphorylation and biophysics of Kv1.3 ion channel by SRC kinase.

3. Immunological responsiveness against tumors induced by avian sarcoma virus: reduced expression of pp60src kinase activity in regressing tumors.

4. Oncogene v-src transforms and establishes embryonic rodent fibroblasts but not diploid human fibroblasts.

5. Replication of hepatitis delta virus RNA in mice after intramuscular injection of plasmid DNA.

6. New procedure for DNA transfection with polycation and dimethyl sulfoxide.

7. v-src induces clonal sarcomas and rapid metastasis following transduction with a replication-defective retrovirus.

8. Polyoma middle T antigen requires cooperation from another gene to express the malignant phenotype in vivo.

9. src-specific immunity in inbred chickens bearing v-src DNA- and RSV-induced tumors.

10. Tumor immunity generated in the course of regression of v-src-induced sarcomas.