Literature DB >> 6330534

New procedure for DNA transfection with polycation and dimethyl sulfoxide.

S Kawai, M Nishizawa.   

Abstract

A new procedure for DNA transfection has been developed in a system of chicken embryo fibroblast cells and cloned Rous sarcoma virus DNA by using a polycation reagent as a mediator to adsorb DNA to the cell surface and dimethyl sulfoxide as an agent to facilitate the uptake of adsorbed DNA by the cells. In this new, simple, and convenient polycation-dimethyl sulfoxide transfection, which requires no carrier DNA even with small amounts of DNA, the number of transformed cell foci induced by Rous sarcoma virus DNA was proportional to the dose of the transfecting DNA, and chicken embryo fibroblast cells were successfully transformed by v-src-containing subgenomic DNA as well.

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Year:  1984        PMID： 6330534      PMCID： PMC368888          DOI： 10.1128/mcb.4.6.1172-1174.1984

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  21 in total

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Authors:  S Kawai; T Yamamoto
Journal:  Jpn J Exp Med       Date:  1970-08

2.  The infectivity of polyoma virus DNA for mouse embryo cells in the presence of diethylaminoethyl-dextran.

Authors:  D Warden; H V Thorne
Journal:  J Gen Virol       Date:  1968-12       Impact factor: 3.891

3.  Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions.

Authors:  K Toyoshima; P K Vogt
Journal:  Virology       Date:  1969-07       Impact factor: 3.616

4.  DEAE-dextran: enhancement of cellular transformation induced by avian sarcoma viruses.

Authors:  P K Vogt
Journal:  Virology       Date:  1967-09       Impact factor: 3.616

5.  Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.

Authors:  J H McCutchan; J S Pagano
Journal:  J Natl Cancer Inst       Date:  1968-08       Impact factor: 13.506

6.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

7.  Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment.

Authors:  G Pontecorvo
Journal:  Somatic Cell Genet       Date:  1975-10

8.  Complementation rescue of Rous sarcoma virus from transformed mammalian cells by polyethylene glycol-mediated cell fusion.

Authors:  K S Steimer; D Boettiger
Journal:  J Virol       Date:  1977-07       Impact factor: 5.103

9.  Infectious DNA from herpes simplex virus: infectivity of double-stranded and single-stranded molecules.

Authors:  P Sheldrick; M Laithier; D Lando; M L Ryhiner
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

10.  An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA.

Authors:  N D Stow; N M Wilkie
Journal:  J Gen Virol       Date:  1976-12       Impact factor: 3.891
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  212 in total

1.  Activation of promoter P4 of the autonomous parvovirus minute virus of mice at early S phase is required for productive infection.

Authors:  L Deleu; A Pujol; S Faisst; J Rommelaere
Journal:  J Virol       Date:  1999-05       Impact factor: 5.103

2.  Psi- vectors: murine leukemia virus-based self-inactivating and self-activating retroviral vectors.

Authors:  K A Delviks; W S Hu; V K Pathak
Journal:  J Virol       Date:  1997-08       Impact factor: 5.103

3.  The v-rel oncogene: insights into the mechanism of transcriptional activation, repression, and transformation.

Authors:  W H Walker; B Stein; P A Ganchi; J A Hoffman; P A Kaufman; D W Ballard; M Hannink; W C Greene
Journal:  J Virol       Date:  1992-08       Impact factor: 5.103

4.  Cooperative effects of C/EBP-like and NF kappa B-like binding sites on rat serum amyloid A1 gene expression in liver cells.

Authors:  X Li; W S Liao
Journal:  Nucleic Acids Res       Date:  1992-09-25       Impact factor: 16.971

5.  Unusually high frequency of reconstitution of long terminal repeats in U3-minus retrovirus vectors by DNA recombination or gene conversion.

Authors:  P Olson; H M Temin; R Dornburg
Journal:  J Virol       Date:  1992-03       Impact factor: 5.103

6.  Addition of N-terminal peptide sequences activates the oncogenic and signaling potentials of the catalytic subunit p110α of phosphoinositide-3-kinase.

Authors:  Minghao Sun; Jonathan R Hart; Petra Hillmann; Marco Gymnopoulos; Peter K Vogt
Journal:  Cell Cycle       Date:  2011-11-01       Impact factor: 4.534

7.  Polyomavirus large T mutants affected in retinoblastoma protein binding are defective in immortalization.

Authors:  A Larose; N Dyson; M Sullivan; E Harlow; M Bastin
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103

8.  v-maf, a viral oncogene that encodes a "leucine zipper" motif.

Authors:  M Nishizawa; K Kataoka; N Goto; K T Fujiwara; S Kawai
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

9.  E- vectors: development of novel self-inactivating and self-activating retroviral vectors for safer gene therapy.

Authors:  J G Julias; D Hash; V K Pathak
Journal:  J Virol       Date:  1995-11       Impact factor: 5.103

10.  A direct role for C/EBP and the AP-I-binding site in gene expression linked to adipocyte differentiation.

Authors:  R Herrera; H S Ro; G S Robinson; K G Xanthopoulos; B M Spiegelman
Journal:  Mol Cell Biol       Date:  1989-12       Impact factor: 4.272
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