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Literature DB >> 6300768

Sequence of a Drosophila DNA segment that functions in Saccharomyces cerevisiae and its regulation by a yeast promoter.

Abstract

We have determined the complete DNA sequence of a segment derived from Drosophila melanogaster that complements a yeast adenine-8 (ade8) mutation. This sequence, in conjunction with transcriptional analysis, predicts that a 206 amino acid sequence accounts for complementation. We have ligated a 1.5 kb promoter segment of the yeast alcohol dehydrogenase I (ADHI) gene just upstream of the open reading frame. This construction results in ADE8+ function that responds to glucose and ethanol in a manner similar to that for the chromosomal ADHI gene, as determined by a simple quantitative color assay for ADE8+ function.

Entities: Chemical Gene Species

Mesh：

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Year: 1983 PMID： 6300768 PMCID： PMC325753 DOI： 10.1093/nar/11.3.789

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

12 in total

6. Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations.

Authors: D Schild; A J Brake; M C Kiefer; D Young; P J Barr
Journal: Proc Natl Acad Sci U S A Date: 1990-04 Impact factor: 11.205

7. Nucleotide divergence of the rp49 gene region between Drosophila melanogaster and two species of the Obscura group of Drosophila.

Authors: C Segarra; M Aguadé
Journal: J Mol Evol Date: 1993-03 Impact factor: 2.395

8. Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.

Authors: S Henikoff; E H Cohen
Journal: Mol Cell Biol Date: 1984-08 Impact factor: 4.272

9. Evidence for a novel glycinamide ribonucleotide transformylase in Escherichia coli.

Authors: P Nygaard; J M Smith
Journal: J Bacteriol Date: 1993-06 Impact factor: 3.490

9 in total

Sequence of a Drosophila DNA segment that functions in Saccharomyces cerevisiae and its regulation by a yeast promoter.

1. Eukaryotic promoters?

2. A new method for sequencing DNA.

3. The accumulation of glycinamide ribotide by ade3 and ade8 mutants of Saccharomyces cerevisiae.

4. Isolation of a gene from Drosophila by complementation in yeast.

5. The ovalbumin gene-sequence of putative control regions.

6. Transformation of yeast by a replicating hybrid plasmid.

7. Expression of a human gene for interferon in yeast.

8. Targeted deletion of a yeast enolase structural gene. Identification and isolation of yeast enolase isozymes.

9. DNA sequencing with chain-terminating inhibitors.

10. Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.

1. Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli.

2. Two Drosophila melanogaster mutations block successive steps of de novo purine synthesis.

Review 3. The yeast expression system for recombinant glycosyltransferases.

4. Multiple purine pathway enzyme activities are encoded at a single genetic locus in Drosophila.

5. Expression of the major heat shock gene of Drosophila melanogaster in Saccharomyces cerevisiae.

6. Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations.

7. Nucleotide divergence of the rp49 gene region between Drosophila melanogaster and two species of the Obscura group of Drosophila.

8. Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.

9. Evidence for a novel glycinamide ribonucleotide transformylase in Escherichia coli.