Literature DB >> 6298604

Expression of hepatitis B virus surface antigen gene in cultured cells by using recombinant plasmid vectors.

A Siddiqui.   

Abstract

By using a new host-vector system, expression of the gene coding for hepatitis B surface antigen has been studied. A subgenomic fragment of cloned hepatitis B viral DNA was inserted into the plasmid vector pSV010. Transfection of COS cells with the recombinant plasmid vector containing hepatitis sequences leads to the synthesis of hepatitis B surface antigen, which is released in the culture medium in the form of 22-nm particles similar to those found in the sera of hepatitis carriers.

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Year:  1983        PMID： 6298604      PMCID： PMC368511          DOI： 10.1128/mcb.3.1.143-146.1983

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  23 in total

1.  Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322.

Authors:  C J Burrell; P Mackay; P J Greenaway; P H Hofschneider; K Murray
Journal:  Nature       Date:  1979-05-03       Impact factor: 49.962

2.  Cloning in Escherichia coli and physical structure of hepatitis B virion DNA.

Authors:  P Charnay; C Pourcel; A Louise; A Fritsch; P Tiollais
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

3.  Cloning and endonuclease mapping of the hepatitis B viral genome.

Authors:  J J Sninsky; A Siddiqui; W S Robinson; S N Cohen
Journal:  Nature       Date:  1979-05-24       Impact factor: 49.962

4.  Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen.

Authors:  P Valenzuela; P Gray; M Quiroga; J Zaldivar; H M Goodman; W J Rutter
Journal:  Nature       Date:  1979-08-30       Impact factor: 49.962

5.  Restriction endonuclease cleavage map and location of unique features of the DNA of hepatitis B virus, subtype adw2.

Authors:  A Siddiqui; F Sattler; W S Robinson
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

6.  Expression of cloned hepatitis B virus DNA in human cell cultures.

Authors:  S Z Hirschman; P Price; E Garfinkel; J Christman; G Acs
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

7.  Biosynthesis of hepatitis B virus surface antigen in Escherichia coli.

Authors:  P Charnay; M Gervais; A Louise; F Galibert; P Tiollais
Journal:  Nature       Date:  1980-08-28       Impact factor: 49.962

8.  Hepatitis B virus genes and their expression in E. coli.

Authors:  M Pasek; T Goto; W Gilbert; B Zink; H Schaller; P MacKay; G Leadbetter; K Murray
Journal:  Nature       Date:  1979-12-06       Impact factor: 49.962

9.  Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli.

Authors:  F Galibert; E Mandart; F Fitoussi; P Tiollais; P Charnay
Journal:  Nature       Date:  1979-10-25       Impact factor: 49.962

10.  Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA.

Authors:  M F Dubois; C Pourcel; S Rousset; C Chany; P Tiollais
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205
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  13 in total

1.  Expression of hepatitis B viral core region in mammalian cells.

Authors:  M J Roossinck; S Jameel; S H Loukin; A Siddiqui
Journal:  Mol Cell Biol       Date:  1986-05       Impact factor: 4.272

2.  In vivo phosphorylation and protein analysis of hepatitis B virus core antigen.

Authors:  M J Roossinck; A Siddiqui
Journal:  J Virol       Date:  1987-04       Impact factor: 5.103

3.  The hepatitis B virus enhancer modulates transcription of the hepatitis B virus surface antigen gene from an internal location.

Authors:  G A Bulla; A Siddiqui
Journal:  J Virol       Date:  1988-04       Impact factor: 5.103

4.  Transcription of hepatitis B virus by RNA polymerase II.

Authors:  L B Rall; D N Standring; O Laub; W J Rutter
Journal:  Mol Cell Biol       Date:  1983-10       Impact factor: 4.272

5.  The human hepatitis B virus enhancer requires trans-acting cellular factor(s) for activity.

Authors:  S Jameel; A Siddiqui
Journal:  Mol Cell Biol       Date:  1986-02       Impact factor: 4.272

6.  Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA.

Authors:  M A Sells; M L Chen; G Acs
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

Review 7.  Revisiting Hepatitis B Virus: Challenges of Curative Therapies.

Authors:  Jianming Hu; Ulrike Protzer; Aleem Siddiqui
Journal:  J Virol       Date:  2019-09-30       Impact factor: 5.103

8.  Expression of hepatitis B antigens with a simian virus 40 vector.

Authors:  H Will; R Cattaneo; E Pfaff; C Kuhn; M Roggendorf; H Schaller
Journal:  J Virol       Date:  1984-05       Impact factor: 5.103

9.  Expression of the hepatitis B virus X gene in mammalian cells.

Authors:  A Siddiqui; S Jameel; J Mapoles
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

10.  Analysis of processing and polyadenylation signals of the hepatitis B virus surface antigen gene by using simian virus 40-hepatitis B virus chimeric plasmids.

Authors:  C C Simonsen; A D Levinson
Journal:  Mol Cell Biol       Date:  1983-12       Impact factor: 4.272
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