| Literature DB >> 6298465 |
M L Dyall-Smith, A A Azad, I H Holmes.
Abstract
Cloned DNA copies of double-stranded RNA segments 7, 8, and 9 of UK bovine rotavirus were nick-translated with [alpha-(32)P]ATP and hybridized to double-stranded RNA of various rotavirus strains which had been separated on long polyacrylamide gels and then transferred to o-aminophenylthioether paper. Specific hybridization of the UK calf clones to the separated RNA segments allowed the corresponding genes of four different rotaviruses to be rapidly determined.Entities:
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Year: 1983 PMID: 6298465 PMCID: PMC255126
Source DB: PubMed Journal: J Virol ISSN: 0022-538X Impact factor: 5.103