Literature DB >> 6294622

Drosophila topoisomerase I: isolation, purification and characterization.

K Javaherian, Y C Tse, J Vega.   

Abstract

We have purified and characterized topoisomerase I from Drosophila melanogaster. The molecular weight of the enzyme is 135,000; 100,000, 90,000, and 65,000 molecular weight products result from degradation of the enzyme. The enzyme relaxes both positive and negative supercoiled DNA. Mg++ is not absolutely required, but stimulates the enzymatic activity considerably.

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Year:  1982        PMID： 6294622      PMCID： PMC326976          DOI： 10.1093/nar/10.21.6945

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  25 in total

1.  Characterization of purified DNA-relaxing enzyme from human tissue culture cells.

Authors:  W Keller
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

2.  An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).

Authors:  J J Champoux; R Dulbecco
Journal:  Proc Natl Acad Sci U S A       Date:  1972-01       Impact factor: 11.205

3.  Interaction of closed circular DNA with intercalative dyes. II. The free energy of superhelix formation in SV40 DNA.

Authors:  W Bauer; J Vinograd
Journal:  J Mol Biol       Date:  1970-02-14       Impact factor: 5.469

4.  Interaction between DNA and an Escherichia coli protein omega.

Authors:  J C Wang
Journal:  J Mol Biol       Date:  1971-02-14       Impact factor: 5.469

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  DNA of bacteriophage PM2: a closed circular double-stranded molecule.

Authors:  R T Espejo; E S Canelo; R L Sinsheimer
Journal:  Proc Natl Acad Sci U S A       Date:  1969-08       Impact factor: 11.205

7.  A simple method for the preparation of large quantities of pure plasmid DNA.

Authors:  G O Humphreys; G A Willshaw; E S Anderson
Journal:  Biochim Biophys Acta       Date:  1975-04-02

8.  Chromosomal proteins of Drosophila embryos.

Authors:  S C Elgin; L E Hood
Journal:  Biochemistry       Date:  1973-11-20       Impact factor: 3.162

9.  An omega protein from Drosophila melanogaster.

Authors:  W A Baase; J C Wang
Journal:  Biochemistry       Date:  1974-10-08       Impact factor: 3.162

10.  A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.

Authors:  J B LePecq; C Paoletti
Journal:  J Mol Biol       Date:  1967-07-14       Impact factor: 5.469
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  14 in total

1.  Cloning and sequencing of Schizosaccharomyces pombe DNA topoisomerase I gene, and effect of gene disruption.

Authors:  T Uemura; K Morino; S Uzawa; K Shiozaki; M Yanagida
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971

2.  A DNA topoisomerase type I from wheat embryo mitochondria.

Authors:  M Echeverria; M T Martin; B Ricard; S Litvak
Journal:  Plant Mol Biol       Date:  1986-11       Impact factor: 4.076

3.  Isolation and distribution of a Drosophila protein preferentially associated with active regions of the genome.

Authors:  B Fleischmann; R Filipski; G Fleischmann
Journal:  Chromosoma       Date:  1989-03       Impact factor: 4.316

4.  Microinjection of anti-topoisomerase I immunoglobulin G into nuclei of Chironomus tentans salivary gland cells leads to blockage of transcription elongation.

Authors:  E Egyházi; E Durban
Journal:  Mol Cell Biol       Date:  1987-12       Impact factor: 4.272

5.  Isolation and distribution of a Drosophila protein preferentially associated with inactive regions of the genome.

Authors:  G Fleischmann; R Filipski; S C Elgin
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

6.  Association of eukaryotic DNA topoisomerase I with nucleosomes and chromosomal proteins.

Authors:  K Javaherian; L F Liu
Journal:  Nucleic Acids Res       Date:  1983-01-25       Impact factor: 16.971

Review 7.  DNA topoisomerase I in oncology: Dr Jekyll or Mr Hyde?

Authors:  A K Larsen; C Gobert
Journal:  Pathol Oncol Res       Date:  1999       Impact factor: 3.201

8.  Isolation and characterization of a gene encoding DNA topoisomerase I in Drosophila melanogaster.

Authors:  T S Hsieh; S D Brown; P Huang; J Fostel
Journal:  Nucleic Acids Res       Date:  1992-12-11       Impact factor: 16.971

9.  Assembly of regularly spaced nucleosome arrays by Drosophila chromatin assembly factor 1 and a 56-kDa histone-binding protein.

Authors:  M Bulger; T Ito; R T Kamakaka; J T Kadonaga
Journal:  Proc Natl Acad Sci U S A       Date:  1995-12-05       Impact factor: 11.205

10.  Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome.

Authors:  G Fleischmann; G Pflugfelder; E K Steiner; K Javaherian; G C Howard; J C Wang; S C Elgin
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205
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