Literature DB >> 1367035

Overproduction of an acetylxylan esterase from the extreme thermophile "Caldocellum saccharolyticum" in Escherichia coli.

E Lüthi1, N B Jasmat, P L Bergquist.   

Abstract

The xynC gene coding for an acetylxylan esterase from the extreme thermophile "Caldocellum saccharolyticum" was overexpressed in Escherichia coli strain RR28 by cloning the gene downstream from the lacZ promoter region of pUC18 (pNZ1447) or downstream from the temperature-inducible lambda pRpL promoters of pJLA602 (pNZ1600). The protein formed high molecular weight aggregates in induced cells of RR28/pNZ1600 but not in RR28/pNZ1447. The enzyme constituted up to 10% of the total cell protein and was located in the cytoplasmic fraction of RR28/pNZ1447. The acetyl esterase was most active at pH 6.0 and 70-75 degrees C with a half-life of 64 h at 70 degrees C and 30 h at 80 degrees C, respectively.

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Year:  1990        PMID： 1367035     DOI： 10.1007/bf00166783

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  17 in total

1.  Overproduction from a cellulase gene with a high guanosine-plus-cytosine content in Escherichia coli.

Authors:  G P O'Neill; D G Kilburn; R A Warren; R C Miller
Journal:  Appl Environ Microbiol       Date:  1986-10       Impact factor: 4.792

2.  Induction of Cellulolytic and Xylanolytic Enzyme Systems in Streptomyces spp.

Authors:  C R Mackenzie; D Bilous; H Schneider; K G Johnson
Journal:  Appl Environ Microbiol       Date:  1987-12       Impact factor: 4.792

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 4.  Multiplicity of beta-1,4-xylanase in microorganisms: functions and applications.

Authors:  K K Wong; L U Tan; J N Saddler
Journal:  Microbiol Rev       Date:  1988-09

Review 5.  Genetics and potential biotechnological applications of thermophilic and extremely thermophilic micro-organisms.

Authors:  P L Bergquist; D R Love; J E Croft; M B Streiff; R M Daniel; W H Morgan
Journal:  Biotechnol Genet Eng Rev       Date:  1987

6.  Inducible expression vectors incorporating the Escherichia coli atpE translational initiation region.

Authors:  B Schauder; H Blöcker; R Frank; J E McCarthy
Journal:  Gene       Date:  1987       Impact factor: 3.688

7.  Expression of the Pseudomonas aeruginosa PAK pilin gene in Escherichia coli.

Authors:  B B Finlay; B L Pasloske; W Paranchych
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490

8.  Pseudomonas aeruginosa mutants affected in anaerobic growth on arginine: evidence for a four-gene cluster encoding the arginine deiminase pathway.

Authors:  C Vander Wauven; A Piérard; M Kley-Raymann; D Haas
Journal:  J Bacteriol       Date:  1984-12       Impact factor: 3.490

9.  A novel cloning vector for the direct selection of recombinant DNA in E. coli.

Authors:  H Hennecke; I Günther; F Binder
Journal:  Gene       Date:  1982-09       Impact factor: 3.688

10.  Bioconversion of hemicellulose: aspects of hemicellulase production by Trichoderma reesei QM 9414 and enzymic saccharification of hemicellulose.

Authors:  R F Dekker
Journal:  Biotechnol Bioeng       Date:  1983-04       Impact factor: 4.530
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  5 in total

1.  Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for a beta-mannanase from the extremely thermophilic bacterium "Caldocellum saccharolyticum".

Authors:  E Lüthi; N B Jasmat; R A Grayling; D R Love; P L Bergquist
Journal:  Appl Environ Microbiol       Date:  1991-03       Impact factor: 4.792

2.  Purification and characterization of two thermostable acetyl xylan esterases from Thermoanaerobacterium sp. strain JW/SL-YS485.

Authors:  W Shao; J Wiegel
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

3.  Characterization of an acetyl xylan esterase from the anaerobic fungus Orpinomyces sp. strain PC-2.

Authors:  D L Blum; X L Li; H Chen; L G Ljungdahl
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

4.  Purification and some characteristics of the acetylxylan esterase from Schizophyllum commune.

Authors:  N Halgasová; E Kutejová; J Timko
Journal:  Biochem J       Date:  1994-03-15       Impact factor: 3.857

Review 5.  Biohydrogen Production by the Thermophilic Bacterium Caldicellulosiruptor saccharolyticus: Current Status and Perspectives.

Authors:  Abraham A M Bielen; Marcel R A Verhaart; John van der Oost; Servé W M Kengen
Journal:  Life (Basel)       Date:  2013-01-17
  5 in total

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