| Literature DB >> 1732198 |
J D Keasling1, B O Palsson, S Cooper.
Abstract
The cell-cycle replication pattern of the R6K plasmid has been investigated by using the membrane-elution technique to produce cells labelled at different times during the division cycle and scintillation counting for quantitative analysis of radioactive plasmid DNA. The high-copy plasmid R6K replicates exponentially in a cell-cycle-independent manner. A mini-R6K plasmid deleted for the ori alpha origin of replication also replicates, exponentially in a cell-cycle-independent manner.Entities:
Mesh:
Year: 1992 PMID: 1732198 PMCID: PMC206197 DOI: 10.1128/jb.174.3.1060-1062.1992
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490