| Literature DB >> 6292044 |
R Di Lauro, S Obici, A M Acquaviva, C G Alvino.
Abstract
Two plasmids containing rat thyroglobulin cDNA sequences have been constructed and characterized. A plasmid with a 500-bp insert (pRT6) was isolated and identified as thyroglobulin-specific on the basis of the tissue specificity of the inserted sequence and of its ability to retain thyroglobulin mRNA on a nitrocellulose filter. The cDNA insert in pRT6 was subsequently used to screen a rat thyroid cDNA library constructed with large cDNA. A plasmid was found containing a 1700-bp insert. The polarity and the fidelity of the insert is demonstrated by S1 mapping.Entities:
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Year: 1982 PMID： 6292044 DOI： 10.1016/0378-1119(82)90196-2
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688