Literature DB >> 6290987

Expression of a X. laevis tRNATyr gene in mammalian cells.

F A Laski, B Alzner-DeWeerd, U L RajBhandary, P A Sharp.   

Abstract

Expression of a X. laevis tRNATyr gene has been studied in mammalian cells. This tRNATyr gene has a 13 base intervening sequence adjacent to its anticodon. A fragment containing the tRNATyr gene was cloned into the late region of SV40. Cells infected with a recombinant virus stock vastly overproduce a tRNATyr that is properly spliced, processed and modified. It was also found that the X. laevis tRNATyr is identical or nearly identical to an endogenous tRNATyr of monkey kidney cells. The possibility of using the X. laevis tRNATyr gene to create an amber suppressor for mammalian cells is discussed.

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Year:  1982        PMID： 6290987      PMCID： PMC321116          DOI： 10.1093/nar/10.15.4609

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  Unexpected occurrence of an aminoacylated nucleoside in mammalian tRNATyr.

Authors:  R Brambilla; H Rogg; M Staehelin
Journal:  Nature       Date:  1976-09-09       Impact factor: 49.962

2.  Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: enhancement of the gag-pol polypeptide with yeast suppressor tRNA.

Authors:  L Philipson; P Andersson; U Olshevsky; R Weinberg; D Baltimore; R Gesteland
Journal:  Cell       Date:  1978-01       Impact factor: 41.582

3.  Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome.

Authors:  R C Mulligan; B H Howard; P Berg
Journal:  Nature       Date:  1979-01-11       Impact factor: 49.962

4.  Efficient correction of a mutation by use of chemically synthesized DNA.

Authors:  A Razin; T Hirose; K Itakura; A D Riggs
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

5.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

6.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

7.  Super-suppressors in Saccharomyces cerevisiae.

Authors:  R A Gilmore
Journal:  Genetics       Date:  1967-08       Impact factor: 4.562

8.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

9.  Mutagenesis at a specific position in a DNA sequence.

Authors:  C A Hutchison; S Phillips; M H Edgell; S Gillam; P Jahnke; M Smith
Journal:  J Biol Chem       Date:  1978-09-25       Impact factor: 5.157

10.  Sequence organization of a cloned tDNA met fragment from Xenopus laevis.

Authors:  S G Clarkson; V Kurer; H O Smith
Journal:  Cell       Date:  1978-07       Impact factor: 41.582
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  25 in total

1.  A transformed human epithelial cell line that retains tight junctions post crisis.

Authors:  A L Cozens; M J Yezzi; M Yamaya; D Steiger; J A Wagner; S S Garber; L Chin; E M Simon; G R Cutting; P Gardner
Journal:  In Vitro Cell Dev Biol       Date:  1992 Nov-Dec

2.  Nuclease Bal-31 mapping of proteins bound to a tRNA(tyr) gene in SV40 minichromosomes.

Authors:  S R Scanlon; W R Folk
Journal:  Nucleic Acids Res       Date:  1991-12       Impact factor: 16.971

3.  Thermal response of rat fibroblasts stably transfected with the human 70-kDa heat shock protein-encoding gene.

Authors:  G C Li; L G Li; Y K Liu; J Y Mak; L L Chen; W M Lee
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

4.  The role of the 5'-flanking sequence of a human tRNA(Glu) gene in modulation of its transcriptional activity in vitro.

Authors:  E S Gonos; J P Goddard
Journal:  Biochem J       Date:  1990-12-15       Impact factor: 3.857

5.  All human tRNATyr genes contain introns as a prerequisite for pseudouridine biosynthesis in the anticodon.

Authors:  H van Tol; H Beier
Journal:  Nucleic Acids Res       Date:  1988-03-25       Impact factor: 16.971

6.  Characterization of yeast mitochondrial RNase P: an intact RNA subunit is not essential for activity in vitro.

Authors:  M J Morales; C A Wise; M J Hollingsworth; N C Martin
Journal:  Nucleic Acids Res       Date:  1989-09-12       Impact factor: 16.971

7.  Efficient expression of small RNA polymerase III genes from a novel simian virus 40 vector and their effect on viral gene expression.

Authors:  R A Bhat; M R Furtado; B Thimmappaya
Journal:  Nucleic Acids Res       Date:  1989-02-11       Impact factor: 16.971

8.  Introduction of an intervening sequence into a human serine suppressor tRNA gene: effects on gene expression in vitro and in vivo.

Authors:  H J Drabkin
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971

9.  Suppression of heat-induced hsp70 expression by the 70-kDa subunit of the human Ku autoantigen.

Authors:  G C Li; S H Yang; D Kim; A Nussenzweig; H Ouyang; J Wei; P Burgman; L Li
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-09       Impact factor: 11.205

10.  Transcriptional activation of human zeta 2 globin promoter by the alpha globin regulatory element (HS-40): functional role of specific nuclear factor-DNA complexes.

Authors:  Q Zhang; P M Reddy; C Y Yu; C Bastiani; D Higgs; G Stamatoyannopoulos; T Papayannopoulou; C K Shen
Journal:  Mol Cell Biol       Date:  1993-04       Impact factor: 4.272
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