Literature DB >> 6287457

Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage at glutamine-glycine pairs.

R Hanecak, B L Semler, C W Anderson, E Wimmer.   

Abstract

Proteolytic processing of poliovirus polypeptides was examined by the addition of antibodies directed against the viral proteins P3-7c and P2-X to a cell-free translation extract prepared from infected HeLa cells. Antisera to P3-7c specifically inhibited in vitro processing at Gln-Gly pairs. Partial amino acid sequence analysis revealed a second Tyr-Gly pair that is utilized in protein processing. Neither Tyr-Gly cleavage is affected by antibody to P3-7c. Anti-P3-7c antibodies react not only with P3-7c but also with P3-6a and P3-2, two viral polypeptides NH2-coterminal with P3-7c. Preimmune and anti-P2-X antibodies had no effect on the processing of poliovirus proteins in vitro. We conclude that the activity responsible for processing poliovirus polypeptides at Gln-Gly pairs resides in the primary structure of P3-7c (or P3-2 and P3-6a) and not in P2-X.

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Year:  1982        PMID： 6287457      PMCID： PMC346558          DOI： 10.1073/pnas.79.13.3973

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  Protease required for processing picornaviral coat protein resides in the viral replicase gene.

Authors:  A C Palmenberg; M A Pallansch; R R Rueckert
Journal:  J Virol       Date:  1979-12       Impact factor: 5.103

2.  Cleavage sites in the polypeptide precursors of poliovirus protein P2-X.

Authors:  B L Semler; R Hanecak; C W Anderson; E Wimmer
Journal:  Virology       Date:  1981-10-30       Impact factor: 3.616

3.  The fate of VPg during in vitro translation of poliovirus RNA.

Authors:  A J Dorner; P G Rothberg; E Wimmer
Journal:  FEBS Lett       Date:  1981-09-28       Impact factor: 4.124

4.  Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.

Authors:  V R Racaniello; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

Review 5.  Structure and expression of the picornavirus genome.

Authors:  N Kitamura; C Adler; E Wimmer
Journal:  Ann N Y Acad Sci       Date:  1980       Impact factor: 5.691

6.  Primary structure, gene organization and polypeptide expression of poliovirus RNA.

Authors:  N Kitamura; B L Semler; P G Rothberg; G R Larsen; C J Adler; A J Dorner; E A Emini; R Hanecak; J J Lee; S van der Werf; C W Anderson; E Wimmer
Journal:  Nature       Date:  1981-06-18       Impact factor: 49.962

7.  Amino-terminal sequence of adenovirus type 2 proteins: hexon, fiber, component IX, and early protein 1B-15K.

Authors:  C W Anderson; J B Lewis
Journal:  Virology       Date:  1980-07-15       Impact factor: 3.616

8.  Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure.

Authors:  J Renart; J Reiser; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

9.  Immunoautoradiographic detection of proteins after electrophoretic transfer from gels to diazo-paper: analysis of adenovirus encoded proteins.

Authors:  J Symington; M Green; K Brackmann
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

10.  Poliovirus replication proteins: RNA sequence encoding P3-1b and the sites of proteolytic processing.

Authors:  B L Semler; C W Anderson; N Kitamura; P G Rothberg; W L Wishart; E Wimmer
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205
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  105 in total

1.  Poliovirus requires a precise 5' end for efficient positive-strand RNA synthesis.

Authors:  J Herold; R Andino
Journal:  J Virol       Date:  2000-07       Impact factor: 5.103

2.  Site-specific mutations at a picornavirus VP3/VP1 cleavage site disrupt in vitro processing and assembly of capsid precursors.

Authors:  G D Parks; A C Palmenberg
Journal:  J Virol       Date:  1987-12       Impact factor: 5.103

3.  Expression of enzymatically active poliovirus RNA-dependent RNA polymerase in Escherichia coli.

Authors:  C D Morrow; B Warren; M R Lentz
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

4.  Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease.

Authors:  J C Carrington; W G Dougherty
Journal:  J Virol       Date:  1987-08       Impact factor: 5.103

5.  Intramolecular and intermolecular uridylylation by poliovirus RNA-dependent RNA polymerase.

Authors:  Oliver C Richards; Jeannie F Spagnolo; John M Lyle; Susan E Vleck; Robert D Kuchta; Karla Kirkegaard
Journal:  J Virol       Date:  2006-08       Impact factor: 5.103

6.  Three poliovirus 2B mutants exhibit noncomplementable defects in viral RNA amplification and display dosage-dependent dominance over wild-type poliovirus.

Authors:  K L Johnson; P Sarnow
Journal:  J Virol       Date:  1991-08       Impact factor: 5.103

7.  Proteinase trapping: screening for viral proteinase mutants by alpha complementation.

Authors:  H D Liebig; T Skern; M Luderer; W Sommergruber; D Blaas; E Kuechler
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-15       Impact factor: 11.205

8.  Similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest common ancestry of these virus families.

Authors:  P Argos; G Kamer; M J Nicklin; E Wimmer
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

9.  Poliovirus protein 3AB forms a complex with and stimulates the activity of the viral RNA polymerase, 3Dpol.

Authors:  S J Plotch; O Palant
Journal:  J Virol       Date:  1995-11       Impact factor: 5.103

10.  Coinfection with recombinant vaccinia viruses expressing poliovirus P1 and P3 proteins results in polyprotein processing and formation of empty capsid structures.

Authors:  D C Ansardi; D C Porter; C D Morrow
Journal:  J Virol       Date:  1991-04       Impact factor: 5.103
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