Literature DB >> 6272282

Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.

V R Racaniello, D Baltimore.   

Abstract

The complete 7410 nucleotide sequence of poliovirus type I genome was obtained from cloned cDNA. Double-stranded poliovirus cDNA was synthesized and inserted into the Pst I site of plasmid pBR322, and three clones were derived that together provided DNA copies of the entire poliovirus genome. Two of the clones contained inserts of 2.5 and 6.5 kilobases and represented all but the 5' 115 bases of poliovirus RNA. A third clone was generated from primer-extended DNA and contained sequences from the 5' end of the viral RNA. An open reading frame that was identified in the nucleotide sequence starting 743 bases from the 5' end of the RNA and extending to a termination codon 71 bases from the 3' end contained known poliovirus polypeptide sequence.

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Year:  1981        PMID： 6272282      PMCID： PMC320284          DOI： 10.1073/pnas.78.8.4887

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  32 in total

1.  Translation of poliovirus RNA in vitro: detection of two different initiation sites.

Authors:  M L Celma; E Ehrenfeld
Journal:  J Mol Biol       Date:  1975-11-15       Impact factor: 5.469

2.  Selective and reversible inhibition of initiation of protein synthesis in mammalian cells.

Authors:  J L Saborio; S S Pong; G Koch
Journal:  J Mol Biol       Date:  1974-05-15       Impact factor: 5.469

3.  Effect of pactamycin on synthesis of poliovirus proteins: a method for genetic mapping.

Authors:  R Taber; D Rekosh; D Baltimore
Journal:  J Virol       Date:  1971-10       Impact factor: 5.103

4.  Expression of animal virus genomes.

Authors:  D Baltimore
Journal:  Bacteriol Rev       Date:  1971-09

5.  The formation of poliovirus proteins.

Authors:  D Baltimore; M F Jacobson; J Asso; A S Huang
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1969

6.  Further evidence on the formation of poliovirus proteins.

Authors:  M F Jacobson; J Asso; D Baltimore
Journal:  J Mol Biol       Date:  1970-05-14       Impact factor: 5.469

7.  Molecular weight of poliovirus ribonucleic acid.

Authors:  N Granboulan; M Girard
Journal:  J Virol       Date:  1969-10       Impact factor: 5.103

8.  Polypeptide cleavages in the formation of poliovirus proteins.

Authors:  M F Jacobson; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1968-09       Impact factor: 11.205

9.  Evidence for large precursor proteins in poliovirus synthesis.

Authors:  D F Summers; J V Maizel
Journal:  Proc Natl Acad Sci U S A       Date:  1968-03       Impact factor: 11.205

10.  Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region.

Authors:  A L Bothwell; M Paskind; M Reth; T Imanishi-Kari; K Rajewsky; D Baltimore
Journal:  Cell       Date:  1981-06       Impact factor: 41.582
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  251 in total

1.  Oligomeric structures of poliovirus polymerase are important for function.

Authors:  S D Hobson; E S Rosenblum; O C Richards; K Richmond; K Kirkegaard; S C Schultz
Journal:  EMBO J       Date:  2001-03-01       Impact factor: 11.598

2.  Replication of poliovirus RNA with complete internal ribosome entry site deletions.

Authors:  Kenneth E Murray; Benjamin P Steil; Allan W Roberts; David J Barton
Journal:  J Virol       Date:  2004-02       Impact factor: 5.103

3.  Molecular basis of antigenic structures of poliovirus: implications for their evolution during morphogenesis.

Authors:  K Wiegers; R Dernick
Journal:  J Virol       Date:  1992-07       Impact factor: 5.103

4.  A poliovirus replicon containing the chloramphenicol acetyltransferase gene can be used to study the replication and encapsidation of poliovirus RNA.

Authors:  N Percy; W S Barclay; M Sullivan; J W Almond
Journal:  J Virol       Date:  1992-08       Impact factor: 5.103

5.  Specific interactions of HeLa cell proteins with proposed translation domains of the poliovirus 5' noncoding region.

Authors:  J R Gebhard; E Ehrenfeld
Journal:  J Virol       Date:  1992-05       Impact factor: 5.103

6.  Structural architecture of an RNA that competitively inhibits RNase L.

Authors:  Amanda Y Keel; Babal Kant Jha; Jeffrey S Kieft
Journal:  RNA       Date:  2011-11-23       Impact factor: 4.942

7.  A temperature-sensitive defect of enterovirus 70 is located at the uridylylation of the genome-linked protein VPg in vitro.

Authors:  N Takeda; K Miyamura; T Takegami; S Yamazaki
Journal:  Virus Genes       Date:  1989-08       Impact factor: 2.332

8.  Similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest common ancestry of these virus families.

Authors:  P Argos; G Kamer; M J Nicklin; E Wimmer
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

9.  A new cis-acting element for RNA replication within the 5' noncoding region of poliovirus type 1 RNA.

Authors:  K Shiroki; T Ishii; T Aoki; M Kobashi; S Ohka; A Nomoto
Journal:  J Virol       Date:  1995-11       Impact factor: 5.103

10.  Poliovirus protein 3AB forms a complex with and stimulates the activity of the viral RNA polymerase, 3Dpol.

Authors:  S J Plotch; O Palant
Journal:  J Virol       Date:  1995-11       Impact factor: 5.103
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