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Literature DB >> 6287007

Isolation and characterization of simian virus 40 early region deletion mutants.

Abstract

We constructed a tsB4/dl884 double-mutant helper virus and used it to isolate two simian virus 40 early region deletion mutants that lack about half of the DNA sequences normally used to encode the large tumor antigen (T). Both mutants make a normal-sized small t antigen, but neither mutant can replicate its DNA in the absence of a T+ helper.

Entities: Disease Species

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Year: 1982 PMID： 6287007 PMCID： PMC256124

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

14 in total

1. Altered patterns of protein synthesis in infection by SV40 mutants.

Authors: P Tegtmeyer
Journal: Cold Spring Harb Symp Quant Biol Date: 1975

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

4. Defective simian virus 40 genomes: isolation and growth of individual clones.

Authors: J E Mertz; P Berg
Journal: Virology Date: 1974-11 Impact factor: 3.616

5. Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors: B Hirt
Journal: J Mol Biol Date: 1967-06-14 Impact factor: 5.469

6. Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.

Authors: J H McCutchan; J S Pagano
Journal: J Natl Cancer Inst Date: 1968-08 Impact factor: 13.506

7. Construction and analysis of viable deletion mutants of simian virus 40.

Authors: T E Shenk; J Carbon; P Berg
Journal: J Virol Date: 1976-05 Impact factor: 5.103

8. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.

Authors: P Tegtmeyer
Journal: J Virol Date: 1972-10 Impact factor: 5.103

9. Cell-free translation of simian virus 40 early messenger RNA coding for viral T-antigen.

Authors: C Prives; E Gilboa; M Revel; E Winocour
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

10. Inactivating and mutagenic effects of nitrosoguanidine on simian virus 40.

Authors: P Tegtmeyer; C Dohan; C Reznikoff
Journal: Proc Natl Acad Sci U S A Date: 1970-07 Impact factor: 11.205

9 in total

1. The simian virus 40 sequences between 0.169 and 0.423 map units are not essential to immortalize early-passage rat embryo cells.

Authors: L Sompayrac; K J Danna
Journal: Mol Cell Biol Date: 1985-05 Impact factor: 4.272

Isolation and characterization of simian virus 40 early region deletion mutants.

1. Altered patterns of protein synthesis in infection by SV40 mutants.

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

3. A new method for sequencing DNA.

4. Defective simian virus 40 genomes: isolation and growth of individual clones.

5. Selective extraction of polyoma DNA from infected mouse cell cultures.

6. Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.

7. Construction and analysis of viable deletion mutants of simian virus 40.

8. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.

9. Cell-free translation of simian virus 40 early messenger RNA coding for viral T-antigen.

10. Inactivating and mutagenic effects of nitrosoguanidine on simian virus 40.

1. The simian virus 40 sequences between 0.169 and 0.423 map units are not essential to immortalize early-passage rat embryo cells.

2. Less than 40% of the simian virus 40 large T-antigen-coding sequence is required for transformation.

3. A simian virus 40 dl884/tsA58 double mutant is temperature sensitive for abortive transformation.

4. Stabilization of the 53,000-dalton nonviral tumor antigen is not required for transformation by simian virus 40.

5. A cis-acting sequence promotes removal of simian virus 40 DNA from the replication pool.

6. A fragment of the simian virus 40 early genome can induce tumors in nude mice.

7. The T/t common region of simian virus 40 large T antigen contains a distinct transformation-governing sequence.

8. Simian virus 40 sequences between 0.168 and 0.424 map units are not required for abortive transformation.

9. Simian virus 40 deletion mutants that transform with reduced efficiency.