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Literature DB >> 6285900

The SalGI restriction endonuclease. Enzyme specificity.

Abstract

We have analysed the kinetics of DNA cleavage in the reaction between the SalGI restriction endonuclease and plasmid pMB9. This reaction is subject to competitive inhibition by DNA sequences outside the SalGI recognition site; we have determined the Km and Vmax. for the reaction of this enzyme at its recognition site and the KI for its interaction at other DNA sequences. We conclude that the specificity of DNA cleavage by the enzyme is only partly determined by the discrimination it shows for binding at its recognition sequence compared with binding to other DNA sequences.

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Year: 1982 PMID： 6285900 PMCID： PMC1158197 DOI： 10.1042/bj2030093

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

10 in total

4 in total

The SalGI restriction endonuclease. Enzyme specificity.

Review 1. Restriction endonucleases in the analysis and restructuring of dna molecules.

2. Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments.

3. The EcoRI restriction endonuclease with bacteriophage lambda DNA. Kinetic studies.

4. The EcoRI restriction endonuclease with bacteriophage lambda DNA. Equilibrium binding studies.

5. Inhibition of Eco RI action by polynucleotides. A characterization of the non-specific binding of the enzyme to DNA.

6. Non-specific binding of restriction endonuclease EcoR1 to DNA.

7. The EcoRI restriction endonuclease, covalently closed DNA and ethidium bromide.

8. The SalGI restriction endonuclease. Mechanism of DNA cleavage.

9. The SalGI restriction endonuclease. Purification and properties.

10. The reactions of the EcoRi and other restriction endonucleases.

1. The SalGI restriction endonuclease. Mechanism of DNA cleavage.

2. The SalGI restriction endonuclease. Purification and properties.

3. Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays.

4. An exocytoplasmic endonuclease with restriction function in Streptomyces antibioticus.