Literature DB >> 6285898

The SalGI restriction endonuclease. Purification and properties.

A Maxwell, S E Halford.   

Abstract

The type II restriction endonuclease SalGI has been purified to near homogeneity. At least 80% of the protein remaining after the final stage of the preparation is SalGI restriction endonuclease; no contaminating nucleases remain detectable. The principal form of the protein under both native and denaturing conditions is a monomer of M(r) about 29000. The optimal conditions for both enzyme stability and enzyme activity have been determined.

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Year:  1982        PMID： 6285898      PMCID： PMC1158195          DOI： 10.1042/bj2030077

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  24 in total

Review 1.  Structures and mechanisms of DNA restriction and modification enzymes.

Authors:  P Modrich
Journal:  Q Rev Biophys       Date:  1979-08       Impact factor: 5.318

2.  Nucleotide sequence specificity of restriction endonucleases.

Authors:  H O Smith
Journal:  Science       Date:  1979-08-03       Impact factor: 47.728

3.  Electrophoresis of DNA in agarose gels. Optimizing separations of conformational isomers of double- and single-stranded DNAs.

Authors:  P H Johnson; L I Grossman
Journal:  Biochemistry       Date:  1977-09-20       Impact factor: 3.162

4.  Restriction endonucleases and modification methylases in bacteria.

Authors:  A H Bingham; T Atkinson
Journal:  Biochem Soc Trans       Date:  1978       Impact factor: 5.407

5.  A new restriction endonuclease from Streptomyces albus G.

Authors:  J R Arrand; P A Myers; R J Roberts
Journal:  J Mol Biol       Date:  1978-01-05       Impact factor: 5.469

Review 6.  Restriction and modification enzymes and their recognition sequences.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1981-01-10       Impact factor: 16.971

7.  A general method for the purification of restriction enzymes.

Authors:  P J Greene; H L Heyneker; F Bolivar; R L Rodriguez; M C Betlach; A A Covarrubias; K Backman; D J Russel; R Tait; H W Boyer
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

8.  Streptomyces albus G mutants defective in the SalGI restriction-modification system.

Authors:  K F Chater; L C Wilde
Journal:  J Gen Microbiol       Date:  1980-02

9.  Purification, properties and specificity of the restriction endonuclease from Bacillus stearothermophilus.

Authors:  C M Clarke; B S Hartley
Journal:  Biochem J       Date:  1979-01-01       Impact factor: 3.857

10.  The reactions of the EcoRi and other restriction endonucleases.

Authors:  S E Halford; N P Johnson; J Grinsted
Journal:  Biochem J       Date:  1979-05-01       Impact factor: 3.857
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  5 in total

1.  The SalGI restriction endonuclease. Mechanism of DNA cleavage.

Authors:  A Maxwell; S E Halford
Journal:  Biochem J       Date:  1982-04-01       Impact factor: 3.857

2.  The SalGI restriction endonuclease. Enzyme specificity.

Authors:  A Maxwell; S E Halford
Journal:  Biochem J       Date:  1982-04-01       Impact factor: 3.857

3.  BspRI restriction endonuclease: cloning, expression in Escherichia coli and sequential cleavage mechanism.

Authors:  Tamás Raskó; András Dér; Eva Klement; Krystyna Slaska-Kiss; Eszter Pósfai; Katalin F Medzihradszky; Daniel R Marshak; Richard J Roberts; Antal Kiss
Journal:  Nucleic Acids Res       Date:  2010-06-29       Impact factor: 16.971

4.  An exocytoplasmic endonuclease with restriction function in Streptomyces antibioticus.

Authors:  C G de los Reyes-Gavilan; J F Aparicio; C Barbes; C Hardisson; J Sanchez
Journal:  J Bacteriol       Date:  1988-03       Impact factor: 3.490

5.  The resolvase protein from the transposon Tn21.

Authors:  S E Halford; S L Jordan; E A Kirkbride
Journal:  Mol Gen Genet       Date:  1985
  5 in total

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