Literature DB >> 6255431

Inhibition of Eco RI action by polynucleotides. A characterization of the non-specific binding of the enzyme to DNA.

J Langowski, A Pingoud, M Goppelt, G Maass.   

Abstract

The cleavage of the plasmid pBR322 by the restriction endonuclease Eco RI has been studied in the presence of various polynucleotides and the double stranded octanucleotide d-(GGAATTCC) in order to clarify whether there is a preferential interaction of Eco RI with DNA sequences other than -GAATTC-. The steady state kinetic analysis shows that all polynucleotides investigated with the possible exception of poly-dG.poly-dC inhibit the cleavage competitively with Ki values in the range of 10(-4) to 10(-5) [M nucleotides]. The Ki of d-(GGAATTCC) is 1.5.10(-6) [M nucleotides], indicating that the specific binding is approx. 2 orders of magnitude stronger than non-specific binding.

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Year:  1980        PMID： 6255431      PMCID： PMC324382          DOI： 10.1093/nar/8.20.4727

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

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2.  Superstructure of linear duplex DNA.

Authors:  H J Vollenweider; T Koller; J Parello; J M Sogo
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4.  Non-specific DNA binding of genome regulating proteins as a biological control mechanism: I. The lac operon: equilibrium aspects.

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5.  Affinity chromatography of phosphofructokinase using Cibacron blue F3G-A.

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6.  Diffusion controlled reaction rates in spheroidal geometry. Application to repressor--operator association and membrane bound enzymes.

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Journal:  Biophys Chem       Date:  1974-10       Impact factor: 2.352

7.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
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8.  EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme.

Authors:  P Modrich; D Zabel
Journal:  J Biol Chem       Date:  1976-10-10       Impact factor: 5.157

9.  Kinetic studies on the cleavage of adenovirus DNA by restriction endonuclease Eco RI.

Authors:  S Forsblom; R Rigler; M Ehrenberg; L Philipson
Journal:  Nucleic Acids Res       Date:  1976-12       Impact factor: 16.971

10.  A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose.

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Journal:  J Biol Chem       Date:  1968-12-10       Impact factor: 5.157
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  10 in total

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2.  Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI.

Authors:  H Wolfes; J Alves; A Fliess; R Geiger; A Pingoud
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Review 3.  Type II restriction endonucleases--a historical perspective and more.

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4.  The effect of several nucleic acid binding drugs on the cleavage of d(GGAATTCC) and pBR 322 by the Eco RI restriction endonuclease.

Authors:  M Goppelt; J Langowski; A Pingoud; W Haupt; C Urbanke; H Mayer; G Maass
Journal:  Nucleic Acids Res       Date:  1981-11-25       Impact factor: 16.971

5.  Transient cleavage kinetics of the Eco RI restriction endonuclease measured in a pulsed quench-flow apparatus: enzyme concentration-dependent activity change.

Authors:  J Langowski; C Urbanke; A Pingoud; G Maass
Journal:  Nucleic Acids Res       Date:  1981-07-24       Impact factor: 16.971

6.  Does the specific recognition of DNA by the restriction endonuclease EcoRI involve a linear diffusion step? Investigation of the processivity of the EcoRI endonuclease.

Authors:  J Langowski; J Alves; A Pingoud; G Maass
Journal:  Nucleic Acids Res       Date:  1983-01-25       Impact factor: 16.971

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Authors:  S E Halford; N P Johnson
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8.  The SalGI restriction endonuclease. Enzyme specificity.

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Journal:  Biochem J       Date:  1982-04-01       Impact factor: 3.857

9.  Single turnovers of the EcoRI restriction endonuclease.

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10.  Identification of protein structural elements responsible for the diversity of sequence preferences among Mini-III RNases.

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Journal:  Sci Rep       Date:  2016-12-07       Impact factor: 4.379
  10 in total

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