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Literature DB >> 6285309

Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.

Abstract

The blood of the frog X.laevis contains 2 albumins of 68,000 and 74,000 daltons which are encoded in the liver by two related mRNAs. When an amplified X.laevis DNA library was screened with cloned albumin cDNA only 68,000 dalton albumin gene sequences were isolated. Hybridisation of the albumin cDNA to Southern-blots of Eco R1 digested X.laevis DNA showed that the sequences present in the recombinants did not account for all the fragments which hybridised on the Southern-blots. This indicated that 74K albumin gene sequences exist but that they are not present in the amplified DNA library. A X.laevis genomic library was therefore constructed and screened for albumin genes without amplification. Both 68K and 74K albumin gene sequences were isolated. Recombinants containing 74K albumin gene sequences grew extremely poorly and this probably explains why the 74K albumin sequences were ot isolated from the amplified library. Characterisation of the cloned DNA indicates that there is one sequence coding for the 68K albumin but two different sequences coding for the 75K albumin.

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Year: 1982 PMID： 6285309 PMCID： PMC320656 DOI： 10.1093/nar/10.9.2791

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

1. The rat serum albumin gene: analysis of cloned sequences.

Authors: T D Sargent; J R Wu; J M Sala-Trepat; R B Wallace; A A Reyes; J Bonner
Journal: Proc Natl Acad Sci U S A Date: 1979-07 Impact factor: 11.205

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

3. Molecular cloning and characterization of the human beta-like globin gene cluster.

Authors: E F Fritsch; R M Lawn; T Maniatis
Journal: Cell Date: 1980-04 Impact factor: 41.582

4. The isolation of structural genes from libraries of eucaryotic DNA.

Authors: T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal: Cell Date: 1978-10 Impact factor: 41.582

5. Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.

Authors: W Wahli; I B Dawid
Journal: Proc Natl Acad Sci U S A Date: 1980-03 Impact factor: 11.205

6. Rapid DNA isolations for enzymatic and hybridization analysis.

Authors: R W Davis; M Thomas; J Cameron; T P St John; S Scherer; R A Padgett
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

7. The structure and evolution of the human beta-globin gene family.

Authors: A Efstratiadis; J W Posakony; T Maniatis; R M Lawn; C O'Connell; R A Spritz; J K DeRiel; B G Forget; S M Weissman; J L Slightom; A E Blechl; O Smithies; F E Baralle; C C Shoulders; N J Proudfoot
Journal: Cell Date: 1980-10 Impact factor: 41.582

8. Comparison of cytoplasmic and nuclear poly(A)-containing RNA sequences in Xenopus liver cells.

Authors: G U Ryffel
Journal: Eur J Biochem Date: 1976-02-16

9. Vitellogenin in Xenopus laevis is encoded in a small family of genes.

Authors: W Wahli; I B Dawid; T Wyler; R B Jaggi; R Weber; G U Ryffel
Journal: Cell Date: 1979-03 Impact factor: 41.582

10. Albumin phylogeny for clawed frogs (Xenopus).

Authors: C A Bisbee; M A Baker; A C Wilson; I Haji-Azimi; M Fischberg
Journal: Science Date: 1977-02-25 Impact factor: 47.728

13 in total

1. Activation of chromosomal vitellogenin genes in Xenopus oocytes by pure estrogen receptor and independent activation of albumin genes.

Authors: E A McKenzie; N A Cridland; J Knowland
Journal: Mol Cell Biol Date: 1990-12 Impact factor: 4.272

Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.

1. The rat serum albumin gene: analysis of cloned sequences.

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

3. Molecular cloning and characterization of the human beta-like globin gene cluster.

4. The isolation of structural genes from libraries of eucaryotic DNA.

5. Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.

6. Rapid DNA isolations for enzymatic and hybridization analysis.

7. The structure and evolution of the human beta-globin gene family.

8. Comparison of cytoplasmic and nuclear poly(A)-containing RNA sequences in Xenopus liver cells.

9. Vitellogenin in Xenopus laevis is encoded in a small family of genes.

10. Albumin phylogeny for clawed frogs (Xenopus).

1. Activation of chromosomal vitellogenin genes in Xenopus oocytes by pure estrogen receptor and independent activation of albumin genes.

2. An empirical method for the evaluation of the quality of genomic DNA libraries.

3. DNA sequence of the first exon and 5' flanking region of the 68-K serum albumin gene of Xenopus laevis.

4. A novel nuclear transcription system which responds correctly to cloned estrogen receptor.

5. Scattering of repetitive DNA sequences in the albumin and vitellogenin gene loci of Xenopus laevis.

6. Mouse mammary tumour virus related sequences are present in human DNA.

7. Vitellogenin B2 gene in Xenopus laevis: isolation, in vitro transcription and relation to other vitellogenin genes.

8. Isolation and characterization of calmodulin genes from Xenopus laevis.

9. Vitellogenin genes A1 and B1 are linked in the Xenopus laevis genome.

10. The primary structure of the larval beta 1-globin gene of Xenopus laevis and its flanking regions.