Literature DB >> 2987863

An empirical method for the evaluation of the quality of genomic DNA libraries.

S L Phillips, N C Casavant, C A Hutchison, M H Edgell.   

Abstract

An empirical method is presented that enables one to determine, with only a relatively small investment of time and materials, whether a lambda genomic DNA library will be a productive source of clones carrying specific sequences. The method provides an indication of the abundance of the sequences in library DNA and indicates whether major DNA rearrangements have occurred during library construction and amplification.

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Year:  1985        PMID： 2987863      PMCID： PMC341188          DOI： 10.1093/nar/13.8.2699

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  Molecular cloning and characterization of the human beta-like globin gene cluster.

Authors:  E F Fritsch; R M Lawn; T Maniatis
Journal:  Cell       Date:  1980-04       Impact factor: 41.582

3.  DNA sequence organization of the beta-globin complex in the BALB/c mouse.

Authors:  C L Jahn; C A Hutchison; S J Phillips; S Weaver; N L Haigwood; C F Voliva; M H Edgell
Journal:  Cell       Date:  1980-08       Impact factor: 41.582

4.  High capacity gel preparative electrophoresis for purification of fragments of genomic DNA.

Authors:  F Polsky; M H Edgell; J G Seidman; P Leder
Journal:  Anal Biochem       Date:  1978-07-01       Impact factor: 3.365

5.  The isolation of structural genes from libraries of eucaryotic DNA.

Authors:  T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal:  Cell       Date:  1978-10       Impact factor: 41.582

6.  Chromosomal arrangement of the chicken beta-type globin genes.

Authors:  M Dolan; B J Sugarman; J B Dodgson; J D Engel
Journal:  Cell       Date:  1981-06       Impact factor: 41.582

7.  Construction of a recombinant bacterial plasmid containing DNA sequences for a mouse embryonic globin chain.

Authors:  A Fantoni; I Bozzoni; E Ullu; M G Farace
Journal:  Nucleic Acids Res       Date:  1979-08-10       Impact factor: 16.971

8.  Structure and organization of the highly repeated and interspersed 1.3 kb EcoRI-Bg1II sequence family in mice.

Authors:  R Heller; N Arnheim
Journal:  Nucleic Acids Res       Date:  1980-11-11       Impact factor: 16.971

9.  Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.

Authors:  F E May; R Weber; B R Westley
Journal:  Nucleic Acids Res       Date:  1982-05-11       Impact factor: 16.971

10.  Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro.

Authors:  F Rougeon; B Mach
Journal:  Gene       Date:  1977-05       Impact factor: 3.688
  10 in total
  3 in total

1.  Extensive movement of LINES ONE sequences in beta-globin loci of Mus caroli and Mus domesticus.

Authors:  N C Casavant; S C Hardies; F D Funk; M B Comer; M H Edgell; C A Hutchison
Journal:  Mol Cell Biol       Date:  1988-11       Impact factor: 4.272

2.  Isolation and characterization of a fruit-specific cDNA and the corresponding genomic clone from tomato.

Authors:  J R Pear; N Ridge; R Rasmussen; R E Rose; C M Houck
Journal:  Plant Mol Biol       Date:  1989-12       Impact factor: 4.076

3.  Mitochondrial DNA of cytoplasmic male-sterile Triticum timopheevi: rearrangement of upstream sequences of the atp6 and orf25 genes.

Authors:  S Mohr; E Schulte-Kappert; W Odenbach; G Oettler; U Kück
Journal:  Theor Appl Genet       Date:  1993-04       Impact factor: 5.699
  3 in total

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