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Literature DB >> 745990

DNA methylation at a CCGG sequence in the large intron of the rabbit beta-globin gene: tissue-specific variations.

Abstract

We have analysed DNA modification in a HapII site (CCGG) present in the major intron of the discontinuous rabbit beta-globin gene. In most somatic tissues, including erythroid and non-erythroid tissues, about 50% of the DNA is resistant to cleavage at this site by HapII, though 100% cleavage is found with the isoschizomer MspI. Since the former enzyme is unable to cleave CCGG sites if the internal C residue is 5-methyl C (and since methylation is the only form of CpG modification documented in animal DNA), while the latter enzyme cleaves DNA irrespective of methylation at this residue, we infer that 50% of the CCGG sites in the beta-globin gene intron are methylated in these tissues. The same site appears to be 100% methylated (judged by the same criterium) in sperm DNA and about 80% methylated in brain DNA. DNA from the rabbit SIRC cell line is entirely unmethylated at this site.

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Year: 1978 PMID： 745990 PMCID： PMC342778 DOI： 10.1093/nar/5.12.4631

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

1. Molecular basis of base substitution hotspots in Escherichia coli.

Authors: C Coulondre; J H Miller; P J Farabaugh; W Gilbert
Journal: Nature Date: 1978-08-24 Impact factor: 49.962

2. DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli.

Authors: T Lindahl; S Ljungquist; W Siegert; B Nyberg; B Sperens
Journal: J Biol Chem Date: 1977-05-25 Impact factor: 5.157

3. Rare bases in animal DNA.

Authors: B F Vanyushin; S G Tkacheva; A N Belozersky
Journal: Nature Date: 1970-03-07 Impact factor: 49.962

4. The structure of the rabbit beta-globin gene.

Authors: R A Flavell; C Waalwijk; A J Jeffreys
Journal: Biochem Soc Trans Date: 1978 Impact factor: 5.407

5. Use of restriction enzymes to study eukaryotic DNA methylation: I. The methylation pattern in ribosomal DNA from Xenopus laevis.

Authors: A P Bird; E M Southern
Journal: J Mol Biol Date: 1978-01-05 Impact factor: 5.469

6. Use of restriction enzymes to study eukaryotic DNA methylation: II. The symmetry of methylated sites supports semi-conservative copying of the methylation pattern.

Authors: A P Bird
Journal: J Mol Biol Date: 1978-01-05 Impact factor: 5.469

6. Changes in methylation of tissue cultured soybean cells detected by digestion with the restriction enzymes HpaII and MspI.

Authors: H Quemada; E J Roth; K G Lark
Journal: Plant Cell Rep Date: 1987-02 Impact factor: 4.570

10. Absence of cytosine methylation at C-C-G-G and G-C-G-C sites in the rDNA coding regions and intervening sequences of Drosophila and the rDNA of other insects.

Authors: P M Rae; R E Steele
Journal: Nucleic Acids Res Date: 1979-07-11 Impact factor: 16.971

DNA methylation at a CCGG sequence in the large intron of the rabbit beta-globin gene: tissue-specific variations.

1. Molecular basis of base substitution hotspots in Escherichia coli.

2. DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli.

3. Rare bases in animal DNA.

4. The structure of the rabbit beta-globin gene.

5. Use of restriction enzymes to study eukaryotic DNA methylation: I. The methylation pattern in ribosomal DNA from Xenopus laevis.

6. Use of restriction enzymes to study eukaryotic DNA methylation: II. The symmetry of methylated sites supports semi-conservative copying of the methylation pattern.

7. MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.

8. The rabbit beta-globin gene contains a large large insert in the coding sequence.

9. Specificity of Hpa II and Hae III DNA methylases.

10. A physical map of the DNA regions flanking the rabbit beta-globin gene.

1. Phenotypic switching in cells transformed with the herpes simplex virus thymidine kinase gene.

2. Replication asynchrony between homologs 15q11.2: cytogenetic evidence for genomic imprinting.

3. Hypermethylation of human DNA sequences in embryonal carcinoma cells and somatic tissues but not in sperm.

4. Methylation of chloroplast DNAs in the life cycle of Chlamydomonas.

Review 5. Social and developmental biology of the myxobacteria.

6. Changes in methylation of tissue cultured soybean cells detected by digestion with the restriction enzymes HpaII and MspI.

Review 7. Principles and challenges of genomewide DNA methylation analysis.

8. Expression of a cloned adenovirus gene is inhibited by in vitro methylation.

9. Isolation and characterization of a sperm-specific gene family in the nematode Caenorhabditis elegans.

10. Absence of cytosine methylation at C-C-G-G and G-C-G-C sites in the rDNA coding regions and intervening sequences of Drosophila and the rDNA of other insects.