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Literature DB >> 2997742

Purification of Mbo II methylase (GAAGmA) from Moraxella bovis: site specific cleavage of DNA at nine and ten base pair sequences.

Abstract

The restriction modification methylase M. Mbo II has been purified using a sensitive oligonucleotide linker assay. The enzyme methylates the Mbo II recognition sequence* GAAGA at adenine to produce GAAGmA. M. Mbo II can be used in conjunction with the methylation dependent restriction endonuclease Dpn I (GmATC) to produce cleavage at the 10 base sequence GAAGATCTTC. When M. Mbo II is used in combination with M. Cla I (ATCGATCGAT), cleavage by Dpn I occurs at the four ten base sequences GAAGATCTTC, GAAGATCGAT, ATCGATCTTC and ATCGATCGAT, which is equivalent to a nine base recognition site. The use of combinations of adenine methylases and Dpn I to generate highly selective DNA cleavages at a variety of sequences up to fourteen base pairs is discussed.

Entities: Chemical Species

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Year: 1985 PMID： 2997742 PMCID： PMC322036 DOI： 10.1093/nar/13.20.7171

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

16 in total

1. The effect of site specific methylation on restriction endonuclease digestion.

Authors: M McClelland; M Nelson
Journal: Nucleic Acids Res Date: 1985 Impact factor: 16.971

2. Restriction and modification enzymes and their recognition sequences.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1985 Impact factor: 16.971

3. Methylated oligonucleotides derived from bacteriophage fd RF-DNA modified in vitro by E. coli B modification methylase.

Authors: H van Ormondt; J A Lautenberger; S Linn; A de Waard
Journal: FEBS Lett Date: 1973-07-01 Impact factor: 4.124

4. Alteration of apparent restriction endonuclease recognition specificities by DNA methylases.

Authors: M Nelson; C Christ; I Schildkraut
Journal: Nucleic Acids Res Date: 1984-07-11 Impact factor: 16.971

5. Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.

Authors: C M Joyce; W S Kelley; N D Grindley
Journal: J Biol Chem Date: 1982-02-25 Impact factor: 5.157

6. Site-specific cleavage of DNA at 8- and 10-base-pair sequences.

Authors: M McClelland; L G Kessler; M Bittner
Journal: Proc Natl Acad Sci U S A Date: 1984-02 Impact factor: 11.205

7. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors: D C Schwartz; C R Cantor
Journal: Cell Date: 1984-05 Impact factor: 41.582

8. Sequence and gene organization of mouse mitochondrial DNA.

Authors: M J Bibb; R A Van Etten; C T Wright; M W Walberg; D A Clayton
Journal: Cell Date: 1981-10 Impact factor: 41.582

9. A DNA methylase from Thermus thermophilus HB8.

Authors: S Sato; K Nakazawa; T Shinomiya
Journal: J Biochem Date: 1980-09 Impact factor: 3.387

10. Purification and characterization of two new modification methylases: MClaI from Caryophanon latum L and MTaqI from Thermus aquaticus YTI.

Authors: M McClelland
Journal: Nucleic Acids Res Date: 1981-12-21 Impact factor: 16.971

18 in total

1. The FokI methyltransferase from Flavobacterium okeanokoites. Purification and characterization of the enzyme and its truncated derivatives.

Authors: T Kaczorowski; M Sektas; P Skowron; A J Podhajska
Journal: Mol Biotechnol Date: 1999-11 Impact factor: 2.695

2. Alw26I, Eco31I and Esp3I--type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA.

Authors: J Bitinaite; Z Maneliene; S Menkevicius; S Klimasauskas; V Butkus; A Janulaitis
Journal: Nucleic Acids Res Date: 1992-10-11 Impact factor: 16.971

Purification of Mbo II methylase (GAAGmA) from Moraxella bovis: site specific cleavage of DNA at nine and ten base pair sequences.

1. The effect of site specific methylation on restriction endonuclease digestion.

2. Restriction and modification enzymes and their recognition sequences.

3. Methylated oligonucleotides derived from bacteriophage fd RF-DNA modified in vitro by E. coli B modification methylase.

4. Alteration of apparent restriction endonuclease recognition specificities by DNA methylases.

5. Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.

6. Site-specific cleavage of DNA at 8- and 10-base-pair sequences.

7. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

8. Sequence and gene organization of mouse mitochondrial DNA.

9. A DNA methylase from Thermus thermophilus HB8.

10. Purification and characterization of two new modification methylases: MClaI from Caryophanon latum L and MTaqI from Thermus aquaticus YTI.

1. The FokI methyltransferase from Flavobacterium okeanokoites. Purification and characterization of the enzyme and its truncated derivatives.

2. Alw26I, Eco31I and Esp3I--type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA.

3. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

4. The mutH gene regulates the replication and methylation of the pMB1 origin.

5. Cloning and characterization of the MboII restriction-modification system.

6. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

7. Restriction enzymes and their isoschizomers.

8. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

9. Restriction enzymes and their isoschizomers.

Review 10. The effect of site-specific methylation on restriction-modification enzymes.