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Literature DB >> 6278401

RSITE: a computer program to predict the recognition sequence of a restriction enzyme.

Abstract

A computer program (RSITE) was developed which predicts the recognition sequence of a restriction endonuclease. The sizes of fragments experimentally determined on cleavage of a DNA of known sequence were input. Possible recognition sequences producing fragments of sizes matching those determined empirically were printed out. The program faithfully predicted the specificity of restriction enzymes of known recognition sequence and also determined the recognition sequence of a new restriction enzyme from Haemophilus influenzae GU (HinGU II).

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Year: 1982 PMID： 6278401 PMCID： PMC326108 DOI： 10.1093/nar/10.1.1

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

1. Nomenclature for incompletely specified bases in nucleic acid sequences. Recommendations 1984. Nomenclature Committee of the International Union of Biochemistry (NC-IUB).

Authors:
Journal: Proc Natl Acad Sci U S A Date: 1986-01 Impact factor: 11.205

6. Isolation and computer-aided characterization of MmeI, a type II restriction endonuclease from Methylophilus methylotrophus.

Authors: A C Boyd; I G Charles; J W Keyte; W J Brammar
Journal: Nucleic Acids Res Date: 1986-07-11 Impact factor: 16.971

7. Restriction enzymes and their isoschizomers.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1988 Impact factor: 16.971

8. Restriction and modification enzymes and their recognition sequences.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1984 Impact factor: 16.971

9. Nomenclature Committee of the International Union of Biochemistry (NC-IUB). Nomenclature for incompletely specified bases in nucleic acid sequences. Recommendations 1984.

Authors:
Journal: Biochem J Date: 1985-07-15 Impact factor: 3.857

9 in total

RSITE: a computer program to predict the recognition sequence of a restriction enzyme.

1. Arthrobacter luteus restriction endonuclease cleavage map of phi chi 174 RF DNA.

2. Mapping of the HhaI and HinfI cleavage sites on simian virus 40 DNA.

3. The genome of simian virus 40.

4. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

5. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.

6. A cleavage map of bacteriophage phiX174 genome.

7. 'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases.

8. The nucleotide sequence of bacteriophage phiX174.

9. A computer assisted method for the determination of restriction enzyme recognifion sites.

10. Sequence data handling by computer.

1. Nomenclature for incompletely specified bases in nucleic acid sequences. Recommendations 1984. Nomenclature Committee of the International Union of Biochemistry (NC-IUB).

2. Restriction enzymes and their isoschizomers.

3. Restriction enzymes and their isoschizomers.

4. Nomenclature for incompletely specified bases in nucleic acid sequences: recommendations 1984.

5. Restriction and modification enzymes and their recognition sequences.

6. Isolation and computer-aided characterization of MmeI, a type II restriction endonuclease from Methylophilus methylotrophus.

7. Restriction enzymes and their isoschizomers.

8. Restriction and modification enzymes and their recognition sequences.

9. Nomenclature Committee of the International Union of Biochemistry (NC-IUB). Nomenclature for incompletely specified bases in nucleic acid sequences. Recommendations 1984.