| Literature DB >> 6277870 |
Abstract
A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicated in both organisms, had unique sites for two restriction endonucleases, conferred ampicillin resistance on both organisms, and could be used as a cloning vector in A. nidulans.Entities:
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Year: 1982 PMID: 6277870 PMCID: PMC220131 DOI: 10.1128/jb.150.1.410-413.1982
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490