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Literature DB >> 3085098

Transformation of the cyanobacterium Anacystis nidulans 6301 with the Escherichia coli plasmid pBR322.

Abstract

Anacystis nidulans 6301 has been transformed in the light to ampicillin resistance with the plasmid pBR322. Permeaplasts prepared by 2-hr treatment of cells with lysozyme and EDTA are transformed with a 50-fold higher efficiency than that observed for cells. beta-Lactamase is present in A. nidulans transformed either with pBR322 or the plasmid pCH1 as evidenced by hydrolysis of the beta-lactam ring of Nitrocefin in extracts of transformants. beta-Lactamase also can be immunoprecipitated from extracts of [35S]methionine-labeled pBR322 transformants and coprecipitates with ribulose-bisphosphate carboxylase. Expression of the carboxylase is apparently amplified in pBR322 transformants as is that for several soluble proteins in pCH1 transformants. Chromosomal DNA per cell is increased about 6-fold after transformation of A. nidulans 6301 with either pBR322 or pCH1. A 4.3-kilobase-pair plasmid can be isolated from pBR322 transformants in addition to the endogenous plasmids pUH24 and pUH25.

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Year: 1986 PMID： 3085098 PMCID： PMC323335 DOI： 10.1073/pnas.83.8.2546

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

25 in total

1. Survey of extrachromosomal DNA found in the filamentous cyanobacteria.

Authors: R D Simon
Journal: J Bacteriol Date: 1978-10 Impact factor: 3.490

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
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3. Length of foreign DNA in chimeric plasmids determines the efficiency of its integration into the chromosome of the cyanobacterium Synechococcus R2.

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4. Photosynthetic properties of permaplasts of anacystis.

Authors: B Ward; J Myers
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5. A new approach for molecular cloning in cyanobacteria: cloning of an Anacystis nidulans met gene using a Tn901-induced mutant.

Authors: N Tandeau de Marsac; W E Borrias; C J Kuhlemeier; A M Castets; G A van Arkel; C A van den Hondel
Journal: Gene Date: 1982-11 Impact factor: 3.688

6. Amplification of a chimeric plasmid carrying an erythromycin-resistance determinant introduced into the genome of Streptococcus pneumoniae.

Authors: H Vasseghi; J P Claverys
Journal: Gene Date: 1983-03 Impact factor: 3.688

7. Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans.

Authors: L A Sherman; P van de Putte
Journal: J Bacteriol Date: 1982-04 Impact factor: 3.490

Transformation of the cyanobacterium Anacystis nidulans 6301 with the Escherichia coli plasmid pBR322.

1. Survey of extrachromosomal DNA found in the filamentous cyanobacteria.

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

3. Length of foreign DNA in chimeric plasmids determines the efficiency of its integration into the chromosome of the cyanobacterium Synechococcus R2.

4. Photosynthetic properties of permaplasts of anacystis.

5. A new approach for molecular cloning in cyanobacteria: cloning of an Anacystis nidulans met gene using a Tn901-induced mutant.

6. Amplification of a chimeric plasmid carrying an erythromycin-resistance determinant introduced into the genome of Streptococcus pneumoniae.

7. Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans.

8. Chromosomal-DNA amplification in Bacillus subtilis.

9. A hybrid plasmid is a stable cloning vector for the cyanobacterium Anacystis nidulans R2.

10. Radioisotopic evidence for the polypeptides associated with photosystem II.

1. Amplified expression of ribulose bisphosphate carboxylase/oxygenase in pBR322-transformants of Anacystis nidulans.

2. Characterization of DNA uptake by the cyanobacterium Anacystis nidulans.

3. Uptake and expression of bacterial and cyanobacterial genes by isolated cucumber etioplasts.

4. Cloned manganese superoxide dismutase reduces oxidative stress in Escherichia coli and Anacystis nidulans.

5. Binding, uptake and expression of foreign DNA by cyanobacteria and isolated etioplasts.

6. Comparative Genomics of Synechococcus elongatus Explains the Phenotypic Diversity of the Strains.

7. From conception to COVID-19: an arduous journey of tribulations of racism and triumphs.

Review 8. Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.