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Literature DB >> 6277504

Deletion of the 3' half of the yeast tRNA-Leu3 gene does not abolish promotor function in vitro.

G Carrara, G Di Segni, A Otsuka, G P Tocchini-Valentini.

Abstract

The promotor function of the 3' and 5' half tRNA sequences in the yeast tRNA-Leu3 gene has been studied by in vitro transcription in Xenopus laevis germinal vesicle (GV) extracts. Truncation of the DNA template within the tRNA intervening sequence by Hpa I abolishes transcription. However, separation of the tRNA gene halves by insertion of a 300 bp DNA fragment at the Hpa I site does not affect the promoter efficiency. Further, the complete sequence of the 3' half of the tRNA is not necessary for promoter function, because removal of the 3' half of the gene by cleavage with Pvu II, within the DNA inserted at the Hpa I site, does not inhibit transcription.

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Year: 1981 PMID： 6277504 DOI： 10.1016/0092-8674(81)90420-7

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

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Cited

13 in total

1. Gene size differentially affects the binding of yeast transcription factor tau to two intragenic regions.

Authors: R E Baker; S Camier; A Sentenac; B D Hall
Journal: Proc Natl Acad Sci U S A Date: 1987-12 Impact factor: 11.205

2. Distinctive sequence organization and functional programming of an Alu repeat promoter.

Authors: C Perez-Stable; T M Ayres; C K Shen
Journal: Proc Natl Acad Sci U S A Date: 1984-09 Impact factor: 11.205

3. Comparative mutational analysis of wild-type and stretched tRNA3(Leu) gene promoters.

Authors: P Fabrizio; A Coppo; P Fruscoloni; P Benedetti; G Di Segni; G P Tocchini-Valentini
Journal: Proc Natl Acad Sci U S A Date: 1987-12 Impact factor: 11.205

4. Some bacterial tRNA genes are transcribed by eukaryotic RNA polymerase III.

Authors: W R Folk; H Hofstetter; M L Birnstiel
Journal: Nucleic Acids Res Date: 1982-11-25 Impact factor: 16.971

5. Generation of long read-through transcripts in vivo and in vitro by deletion of 3' termination and processing sequences in the human tRNAimet gene.

Authors: S Adeniyi-Jones; P H Romeo; M Zasloff
Journal: Nucleic Acids Res Date: 1984-01-25 Impact factor: 16.971

Deletion of the 3' half of the yeast tRNA-Leu3 gene does not abolish promotor function in vitro.

1. Gene size differentially affects the binding of yeast transcription factor tau to two intragenic regions.

2. Distinctive sequence organization and functional programming of an Alu repeat promoter.

3. Comparative mutational analysis of wild-type and stretched tRNA3(Leu) gene promoters.

4. Some bacterial tRNA genes are transcribed by eukaryotic RNA polymerase III.

5. Generation of long read-through transcripts in vivo and in vitro by deletion of 3' termination and processing sequences in the human tRNAimet gene.

6. Transcription of tRNA gene fragments by HeLa cell extracts.

7. Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.

8. In vitro binding to the leucine tRNA gene identifies a novel yeast homeobox gene.

9. The role of non-coding DNA sequences in transcription and processing of a yeast tRNA.

10. Specific interactions of Saccharomyces cerevisiae proteins with a promoter region of eukaryotic tRNA genes.