Literature DB >> 6273807

DNA-like duplexes with repetitions. III. Efficient template-guided chemical polymerization of d(TGGCCAAGCTp).

Z A Shabarova, N G Dolinnaya, V L Drutsa, N P Melnikova, A A Purmal.   

Abstract

Self-association of a decanucleotide d(TGGCCAAGCTp) in an aqueous solution is shown by UV spectroscopy, CD and sedimentation analysis to yield a pseudopolymeric (concatemeric) duplex having a geometry similar to that of DNA B-type. It is demonstrated that in conditions when the concatemeric duplex is stable a water-soluble carbodiimide induces efficient polymerization of the 3'- or 5'-phosphorylated decanucleotide, and the resulting polymers d(TGGCCAAGCTp)2-10 contain only natural phosphodiester bonds. In conditions optimal for template-guided polymerization of d(TGGCCAAGCTp) the overall yield of 20-100-member polynucleotides exceeds 90%. The obtained polymeric duplexes are cleaved by restriction endonuclease Alu II, Bsu RI, and Hind III to corresponding decamers which were isolated and sequenced.

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Year:  1981        PMID： 6273807      PMCID： PMC327558          DOI： 10.1093/nar/9.21.5747

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

Authors:  F W STUDIER
Journal:  J Mol Biol       Date:  1965-02       Impact factor: 5.469

2.  Stability of ribonucleic acid double-stranded helices.

Authors:  P N Borer; B Dengler; I Tinoco; O C Uhlenbeck
Journal:  J Mol Biol       Date:  1974-07-15       Impact factor: 5.469

3.  Characterization of a highly repetitive sequence DNA family in rat.

Authors:  L Sealy; J Hartley; J Donelson; R Chalkley; N Hutchison; B Hamkalo
Journal:  J Mol Biol       Date:  1981-01-15       Impact factor: 5.469

4.  Self-complementary oligoribonucleotides: adenylic acid-uridylic acid block copolymers.

Authors:  F H Martin; O C Uhlenbeck; P Doty
Journal:  J Mol Biol       Date:  1971-04-28       Impact factor: 5.469

5.  The application of solid phase method-synthesized oligodeoxyribonucleotides to molecular biology problems.

Authors:  Z A Shabarova
Journal:  Nucleic Acids Symp Ser       Date:  1980

6.  Restriction endonuclease analysis and nucleotide composition of satellite III DNA from Drosophila virilis.

Authors:  D R Kirsch; E H Cohen
Journal:  Biochem Biophys Res Commun       Date:  1980-10-31       Impact factor: 3.575

7.  A simple method for the computation of first neighbour frequencies of DNAs from CD spectra.

Authors:  C Marck; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1978-06       Impact factor: 16.971

8.  DNA-like duplexes with repetitions. I. Properties of concatemer duplexes formed by d(T-G-C-A-C-A-T-G).

Authors:  Z A Shabarova; N G Dolinnaya; S I Turkin; E S Gromova
Journal:  Nucleic Acids Res       Date:  1980-06-11       Impact factor: 16.971

9.  Structure-independent nucleotide sequence analysis.

Authors:  D R Mills; F R Kramer
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

10.  Studies on some interactions and reactions of oligonucleotides in aqueous solution.

Authors:  R Naylor; P T Gilham
Journal:  Biochemistry       Date:  1966-08       Impact factor: 3.162
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  13 in total

1.  Oligonucleotides with conjugated dihydropyrroloindole tripeptides: base composition and backbone effects on hybridization.

Authors:  I V Kutyavin; E A Lukhtanov; H B Gamper; R B Meyer
Journal:  Nucleic Acids Res       Date:  1997-09-15       Impact factor: 16.971

2.  A new affinity reagent for the site-specific, covalent attachment of DNA to active-site nucleophiles: application to the EcoRI and RsrI restriction and modification enzymes.

Authors:  A A Purmal; Z A Shabarova; R I Gumport
Journal:  Nucleic Acids Res       Date:  1992-07-25       Impact factor: 16.971

3.  Evidence of liquid crystal-assisted abiotic ligation of nucleic acids.

Authors:  Tommaso P Fraccia; Giuliano Zanchetta; Valeria Rimoldi; Noel A Clark; Tommaso Bellini
Journal:  Orig Life Evol Biosph       Date:  2015-05-15       Impact factor: 1.950

4.  Ligation with T4 RNA ligase of an oligodeoxyribonucleotide to covalently-linked cross-sectional base-pair analogues of short, normal, and long dimensions.

Authors:  A Petric; B Bhat; N J Leonard; R I Gumport
Journal:  Nucleic Acids Res       Date:  1991-02-11       Impact factor: 16.971

5.  Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. V. Study of single-strand cleavages.

Authors:  A A Yolov; E S Gromova; E A Kubareva; V K Potapov; Z A Shabarova
Journal:  Nucleic Acids Res       Date:  1985-12-20       Impact factor: 16.971

6.  Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. VI. The binding and cleavage of substrates containing nucleotide analogs.

Authors:  A A Yolov; M N Vinogradova; E S Gromova; A Rosenthal; D Cech; V P Veiko; V G Metelev; V G Kosykh; Y I Buryanov; A A Bayev
Journal:  Nucleic Acids Res       Date:  1985-12-20       Impact factor: 16.971

7.  The use of BrCN for assembling modified DNA duplexes and DNA-RNA hybrids; comparison with water-soluble carbodiimide.

Authors:  N G Dolinnaya; N I Sokolova; D T Ashirbekova; Z A Shabarova
Journal:  Nucleic Acids Res       Date:  1991-06-11       Impact factor: 16.971

8.  Site-directed modification of DNA duplexes by chemical ligation.

Authors:  N G Dolinnaya; N I Sokolova; O I Gryaznova; Z A Shabarova
Journal:  Nucleic Acids Res       Date:  1988-05-11       Impact factor: 16.971

9.  Catalytic site-specific cleavage of a DNA-target by an oligonucleotide carrying bleomycin A5.

Authors:  D S Sergeyev; T S Godovikova; V F Zarytova
Journal:  Nucleic Acids Res       Date:  1995-11-11       Impact factor: 16.971

10.  Universal solid supports for the synthesis of oligonucleotides with terminal 3'-phosphates.

Authors:  W T Markiewicz; T K Wyrzykiewicz
Journal:  Nucleic Acids Res       Date:  1989-09-25       Impact factor: 16.971
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