Literature DB >> 2798088

Universal solid supports for the synthesis of oligonucleotides with terminal 3'-phosphates.

W T Markiewicz1, T K Wyrzykiewicz.   

Abstract

The preparation of two types of supports based on controlled pore glass (CPG) is presented. These supports are compatible with established phosphoroamidite chemistry of oligonucleotides synthesis giving rise to an oligonucleotide with terminal 3'-phosphate function during final deprotection. CPG was modified with: (i) methacrylic acid derivatives and 2-mercaptoethanol (1) or (ii) aminoalkylsilane, succinic anhydride and benzidine (2). Support 2 can be also used for the synthesis of partially protected oligonucleotide 3'-phosphates.

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Year:  1989        PMID： 2798088      PMCID： PMC334794          DOI： 10.1093/nar/17.18.7149

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  STUDIES ON POLYNUCLEOTIDES. 43. THE SYNTHESIS OF DEOXRIBOPOLYNUCLEOTIDES CONTAINING REPEATING DINUCLEOTIDE SEQUENCES.

Authors:  E OHTSUKA; M W MOON; H G KHORANA
Journal:  J Am Chem Soc       Date:  1965-07-05       Impact factor: 15.419

2.  Synthesis of 3'-phosphates of diribonucleoside monophosphates via phosphotriester intermediates.

Authors:  J H van Boom; P M Burgers; C A Haasnoot
Journal:  Nucleic Acids Res       Date:  1976-10       Impact factor: 16.971

3.  [Chemical reactions in double-stranded nucleic acids. IV. Cyanogen bromide--a highly effective reagent in the condensation of oligodeoxyribonucleotides].

Authors:  N I Sokolova; D T Ashirbekova; N G Dolinnaia; Z A Shabarova
Journal:  Bioorg Khim       Date:  1987-09

4.  [A new type of DNA modification. Specific introduction of 3'-5'-pyrophosphate internucleotide bonds].

Authors:  A A Purmal'; V L Drutsa; Z A Shabarova
Journal:  Bioorg Khim       Date:  1984-03

5.  Use of ribonucleosides as protecting groups in synthesis of polynucleotides with phosphorylated terminals.

Authors:  J G Nadeau; C K Singleton; G B Kelly; H L Weith; G R Gough
Journal:  Biochemistry       Date:  1984-12-04       Impact factor: 3.162

6.  Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.

Authors:  V A Efimov; A A Buryakova; S V Reverdatto; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971

7.  Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.

Authors:  N D Sinha; J Biernat; J McManus; H Köster
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

8.  DNA-like duplexes with repetitions. III. Efficient template-guided chemical polymerization of d(TGGCCAAGCTp).

Authors:  Z A Shabarova; N G Dolinnaya; V L Drutsa; N P Melnikova; A A Purmal
Journal:  Nucleic Acids Res       Date:  1981-11-11       Impact factor: 16.971

9.  Simple and rapid procedure for synthesis of deoxynucleoside 3'-2-cyanoethyl-N,N-diisopropyl-amino phosphites.

Authors:  A Kraszewski; K E Norris
Journal:  Nucleic Acids Symp Ser       Date:  1987

10.  Synthesis and characterization of oligodeoxyribonucleotides containing terminal phosphates. NMR, UV spectroscopic and thermodynamic analysis of duplex formation of [d(pGGAATTCC)]2, [d(GGAATTCCp)]2 and [d(pGGAATTCCp)]2.

Authors:  M Bower; M F Summers; B Kell; J Hoskins; G Zon; W D Wilson
Journal:  Nucleic Acids Res       Date:  1987-04-24       Impact factor: 16.971
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