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Literature DB >> 6273805

The high affinity binding site on polyoma virus DNA for the viral large-T protein.

Abstract

In order to map the high affinity binding site for the viral large-T protein on polyoma virus DNA, we have developed an assay which does not require purified protein. It is based on the specific elution of the large-T ATPase activity from calf thymus DNA cellulose by recombinant DNA molecules including known sequences of the viral DNA. Using this assay, a high affinity binding site has been mapped on the early region side of the ori region. Binding requires the integrity of a sequence /AGAGGC/TTCC/AGAGGC/ (nucleotides 49 to 64 in the DNA sequence of the A2 strain). Similar repeats of a PuGPuGGC sequence within less than 20 bases are not found within the viral coding regions, but are strikingly common in the control regions of papovaviruses and other eukaryotic DNAs.

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Year: 1981 PMID： 6273805 PMCID： PMC327554 DOI： 10.1093/nar/9.21.5697

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

24 in total

3. Deletion analysis of the polyomavirus late promoter: evidence for both positive and negative elements in the absence of early proteins.

Authors: K B Cahill; G G Carmichael
Journal: J Virol Date: 1989-09 Impact factor: 5.103

The high affinity binding site on polyoma virus DNA for the viral large-T protein.

1. Complementation between BK human papovavirus and a simian virus 40 tsA mutant.

2. Virus-specific early RNA in 3T6 cells infected by a tsA mutant of polyoma virus.

3. The binding site on SV40 DNA for a T antigen-related protein.

4. Polyoma DNA: a physical map.

5. Location of the origin and terminus of replication in polyoma virus DNA.

6. The replication of polyoma DNA.

7. Complementation and transformation by temperature-sensitive mutants of polyoma virus.

8. In vivo sequence requirements of the SV40 early promotor region.

9. Characterization of polyoma virus T antigen.

10. (Na+, K+)-activated adenosinetriphosphatase of axonal membranes, cooperativity and control. Steady-state analysis.

1. PEA1 and PEA3 enhancer elements are primary components of the polyomavirus late transcription initiator element.

2. Replication-dependent transactivation of the polyomavirus late promoter.

3. Deletion analysis of the polyomavirus late promoter: evidence for both positive and negative elements in the absence of early proteins.

4. Small and middle T antigens contribute to lytic and abortive polyomavirus infection.

5. Sequences in the polyomavirus DNA regulatory region involved in viral DNA replication and early gene expression.

6. Requirements for species-specific papovavirus DNA replication.

7. Polyomavirus and simian virus 40 large T antigens bind to common DNA sequences.

8. T-antigen-independent replication of polyomavirus DNA in murine embryonal carcinoma cells.

9. Construction and functional characterization of polyomavirus genomes that separately encode the three early proteins.

10. Essential nucleotides in the polyomavirus origin region.