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Literature DB >> 6273802

The nucleotide sequence of the promoter and the amino-terminal region of alkaline phosphatase structural gene (phoA) of Escherichia coli.

Y Kikuchi, K Yoda, M Yamasaki, G Tamura.

Abstract

The promoter and the amino-terminal region of phoA, the structural gene for alkaline phosphatase of Escherichia coli K12, was cloned by using a promoter cloning vector pMC1403. The nucleotide sequence of the cloned fragment has been determined. A sequence encoding the amino-terminal portion of mature alkaline phosphatase is found and it is preceded by a sequence encoding the signal peptide. The signal peptide consists of 21 amino acids; Met-Lys-Gln-Ser-Thr-Ile-Ala-Leu-Ala-Leu-Leu-Pro-Leu-Leu-Phe-Thr-Pro-Val-Thr-Lys-Ala. The translation initiation codon is GUG, which is preceded by the Shine-Dalgarno sequence GGAG. Upstream to these sequences, there is a typical procaryotic promoter. TATAGTC for the Pribnow box. Around the Pribnow box, there are several dyad symmetrical sequences, which may probably be concerned with the regulation of this gene.

Entities: Gene Species

Mesh：

Substances：

Year: 1981 PMID： 6273802 PMCID： PMC327551 DOI： 10.1093/nar/9.21.5671

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

27 in total

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Journal: Mol Gen Genet Date: 1975

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Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

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Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

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37 in total

1. Identification and characterization of novel phosphate regulon genes, ecs0540-ecs0544, in Escherichia coli O157:H7.

Authors: Yusuke Yoshida; Shinichiro Sugiyama; Tomoya Oyamada; Katsushi Yokoyama; Kozo Makino
Journal: Mol Genet Genomics Date: 2010-07-17 Impact factor: 3.291

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Authors: Yusuke Yoshida; Shinichiro Sugiyama; Tomoya Oyamada; Katsushi Yokoyama; Soo-Ki Kim; Kozo Makino
Journal: J Microbiol Date: 2011-05-03 Impact factor: 3.422

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Authors: Y Sakakibara; K Tsutsumi; K Nakamura; K Yamane
Journal: J Bacteriol Date: 1993-07 Impact factor: 3.490