Literature DB >> 323853

Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro.

H Inouye, J Beckwith.   

Abstract

Alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] of E. coli was synthesized in a cell-free system, and the size of the direct translation product was analyzed. The product has a higher molecular weight than the mature alkaline phosphatase found in the periplasm. The direct translation product can be processed to the mature size by an E. coli membrane fraction; the processing activity copurifies with the outer-membrane fraction. The presumed precursor can dimerize to form active enzyme without being processed, and the resultant enzyme appears to be more hydrophobic than the mature enzyme. These findings are discussed in connection with the "signal hypothesis" proposed for the excretion of proteins across membranes.

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Year:  1977        PMID： 323853      PMCID： PMC430790          DOI： 10.1073/pnas.74.4.1440

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  24 in total

1.  RELEASE OF ALKALINE PHOSPHATASE FROM CELLS OF ESCHERICHIA COLI UPON LYSOZYME SPHEROPLAST FORMATION.

Authors:  M H MALAMY; B L HORECKER
Journal:  Biochemistry       Date:  1964-12       Impact factor: 3.162

Review 2.  In vitro synthesis of protein in microbial systems.

Authors:  G Zubay
Journal:  Annu Rev Genet       Date:  1973       Impact factor: 16.830

3.  Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

Authors:  M J Osborn; J E Gander; E Parisi; J Carson
Journal:  J Biol Chem       Date:  1972-06-25       Impact factor: 5.157

4.  Coordinate and differential in vitro syntheses of two RNA polymerase subunits.

Authors:  S Austin
Journal:  Nature       Date:  1974-12-13       Impact factor: 49.962

5.  Areas of adhesion between wall and membrane of Escherichia coli.

Authors:  M E Bayer
Journal:  J Gen Microbiol       Date:  1968-10

6.  A possible precursor of immunoglobulin light chains.

Authors:  C Milstein; G G Brownlee; T M Harrison; M B Mathews
Journal:  Nat New Biol       Date:  1972-09-27

7.  Hydrocarbon-coated sepharoses. Use in the purification of glycogen phosphorylase.

Authors:  Z Er-el; Y Zaidenzaig; S Shaltiel
Journal:  Biochem Biophys Res Commun       Date:  1972-10-17       Impact factor: 3.575

8.  Isolation and identification of the messenger ribonucleic acid for a structural lipoprotein of the Escherichia coli outer membrane.

Authors:  K Takeishi; M Yasumura; R Pirtle; M Inouye
Journal:  J Biol Chem       Date:  1976-10-25       Impact factor: 5.157

9.  Biologically and chemically pure mRNA coding for a mouse immunoglobulin L-chain prepared with the aid of antibodies and immobilized oligothymidine.

Authors:  I Schechter
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205

10.  Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA.

Authors:  A Hirashima; S Wang; M Inouye
Journal:  Proc Natl Acad Sci U S A       Date:  1974-10       Impact factor: 11.205
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  82 in total

1.  Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.

Authors:  P J Bassford; T J Silhavy; J R Beckwith
Journal:  J Bacteriol       Date:  1979-07       Impact factor: 3.490

2.  Cell envelope alterations in antibiotic-sensitive and-resistant strains of Neisseria gonorrhoeae.

Authors:  L F Guymon; D L Walstad; P F Sparling
Journal:  J Bacteriol       Date:  1978-10       Impact factor: 3.490

3.  Mutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein.

Authors:  S D Emr; M Schwartz; T J Silhavy
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

4.  Interaction of secreted nascent chains with surrounding membrane in Bacillus subtilis.

Authors:  W P Smith; P C Tai; B D Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

5.  Preferential sensitivity of syntheses of exported proteins to translation inhibitors of low polarity in Escherichia coli.

Authors:  M Piovant; S Varenne; J M Pagès; C Lazdunski
Journal:  Mol Gen Genet       Date:  1978-09-08

Review 6.  The sec and prl genes of Escherichia coli.

Authors:  K L Bieker; G J Phillips; T J Silhavy
Journal:  J Bioenerg Biomembr       Date:  1990-06       Impact factor: 2.945

7.  Synthesis and assembly of membrane glycoproteins: presence of leader peptide in nonglycosylated precursor of membrane glycoprotein of vesicular stomatitis virus.

Authors:  R A Irving; F Toneguzzo; S H Rhee; T Hofmann; H P Ghosh
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

8.  Erv26p directs pro-alkaline phosphatase into endoplasmic reticulum-derived coat protein complex II transport vesicles.

Authors:  Catherine A Bue; Christine M Bentivoglio; Charles Barlowe
Journal:  Mol Biol Cell       Date:  2006-09-06       Impact factor: 4.138

9.  Construction, expression, and localization of a CycA::PhoA fusion protein in Rhodobacter sphaeroides and Escherichia coli.

Authors:  A R Varga; S Kaplan
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

10.  New pleiotropic alkaline phosphatase-negative mutants of Escherichia coli K-12.

Authors:  M Heyde; R Portalier
Journal:  J Bacteriol       Date:  1982-08       Impact factor: 3.490
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