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Literature DB >> 6272223

Requirement for the C-terminal region of middle T-antigen in cellular transformation by polyoma virus.

Abstract

Deletions of polyoma virus DNA around the region that codes for the C-terminus of the viral middle T-antigen were created using a transforming fragment (BamH I/EcoR I) of viral DNA cloned in the plasmid vector pAT153. These species were recloned and assayed for their ability to transform Rat-1 cells in culture. Our results showed that whereas the DNA sequence between the presumed translational termination codon for the viral middle T-antigen and the single viral EcoR I site could be removed with no apparent effect on transformation, the removal of the termination codon itself or any amino acid coding sequences of this protein caused a drastic decrease in the transforming ability of the DNA. Transfection of Rat-1 cells with plasmids that contained viral DNA with deletions which corresponded to the last fourteen or more amino acids of the middle T-antigen never gave rise to cellular transformation.

Entities: Disease Species

Mesh：

Substances：

Year: 1981 PMID： 6272223 PMCID： PMC326826 DOI： 10.1093/nar/9.9.2055

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

31 in total

1. CELL-TRANSFORMING ABILITY OF A TEMPERATURE-SENSITIVE MUTANT OF POLYOMA VIRUS.

Authors: M FRIED
Journal: Proc Natl Acad Sci U S A Date: 1965-03 Impact factor: 11.205

2. Virus-specific early RNA in 3T6 cells infected by a tsA mutant of polyoma virus.

Authors: B Cogen
Journal: Virology Date: 1978-03 Impact factor: 3.616

3. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

4. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

Authors: J G Sutcliffe
Journal: Nucleic Acids Res Date: 1978-08 Impact factor: 16.971

5. Polyoma DNA: a physical map.

Authors: B E Griffin; M Fried; A Cowie
Journal: Proc Natl Acad Sci U S A Date: 1974-05 Impact factor: 11.205

6. Host range mutants of polyoma virus.

Authors: T L Benjamin
Journal: Proc Natl Acad Sci U S A Date: 1970-09 Impact factor: 11.205

7. Extracellular nucleases of Pseudomonas BAL 31. I. Characterization of single strand-specific deoxyriboendonuclease and double-strand deoxyriboexonuclease activities.

Authors: H B Gray; D A Ostrander; J L Hodnett; R J Legerski; D L Robberson
Journal: Nucleic Acids Res Date: 1975-09 Impact factor: 16.971

8. 5-Bromo-2'-deoxyuridine potentiation of transformation of rat-embryo cells induced in vitro by 3-methylcholanthrene: induction of rat leukemia virus gs antigen in transformed cells.

Authors: A E Freeman; R V Gilden; M L Vernon; R G Wolford; P E Hugunin; R J Huebner
Journal: Proc Natl Acad Sci U S A Date: 1973-08 Impact factor: 11.205

9. Localization of gene functions in polyoma virus DNA.

Authors: J Feunteun; L Sompayrac; M Fluck; T Benjamin
Journal: Proc Natl Acad Sci U S A Date: 1976-11 Impact factor: 11.205

10. Virus-specific proteins in the plasma membrane of cells lytically infected or transformed by pol-oma virus.

Authors: Y Ito; J R Brocklehurst; R Dulbecco
Journal: Proc Natl Acad Sci U S A Date: 1977-10 Impact factor: 11.205

15 in total

1. Functional asymmetry of the regions juxtaposed to the membrane-binding sequence of polyomavirus middle T antigen.

Authors: J Dahl; U Thathamangalam; R Freund; T L Benjamin
Journal: Mol Cell Biol Date: 1992-11 Impact factor: 4.272

2. Expression of biologically active middle T antigen of polyoma virus from recombinant baculoviruses.

Authors: J Forstová; N Krauzewicz; B E Griffin
Journal: Nucleic Acids Res Date: 1989-02-25 Impact factor: 16.971

Review 3. Lessons in signaling and tumorigenesis from polyomavirus middle T antigen.

Authors: Michele M Fluck; Brian S Schaffhausen
Journal: Microbiol Mol Biol Rev Date: 2009-09 Impact factor: 11.056

4. In vitro mutagenesis of the putative membrane-binding domain of polyomavirus middle-T antigen.

Authors: W Markland; S H Cheng; B A Oostra; A E Smith
Journal: J Virol Date: 1986-07 Impact factor: 5.103

5. DNA sequences required for specific and efficient initiation of transcription at the polyoma virus early promoter.

Authors: P Jat; U Novak; A Cowie; C Tyndall; R Kamen
Journal: Mol Cell Biol Date: 1982-07 Impact factor: 4.272

6. Carboxy terminus of polyoma middle-sized tumor antigen is required for attachment to membranes, associated protein kinase activities, and cell transformation.

Authors: G G Carmichael; B S Schaffhausen; D I Dorsky; D B Oliver; T L Benjamin
Journal: Proc Natl Acad Sci U S A Date: 1982-06 Impact factor: 11.205

7. Characterization of viable mutants of polyomavirus cold sensitive for maintenance of cell transformation.

Authors: D Templeton; W Eckhart
Journal: J Virol Date: 1984-03 Impact factor: 5.103

8. Construction and expression of a recombinant DNA gene encoding a polyomavirus middle-size tumor antigen with the carboxyl terminus of the vesicular stomatitis virus glycoprotein G.

Authors: D Templeton; A Voronova; W Eckhart
Journal: Mol Cell Biol Date: 1984-02 Impact factor: 4.272

9. Transformation by polyoma virus is drastically reduced by substitution of phenylalanine for tyrosine at residue 315 of middle-sized tumor antigen.

Authors: G Carmichael; B S Schaffhausen; G Mandel; T J Liang; T L Benjamin
Journal: Proc Natl Acad Sci U S A Date: 1984-02 Impact factor: 11.205

10. A frameshift mutation affecting the carboxyl terminus of the simian virus 40 large tumor antigen results in a replication- and transformation-defective virus.

Authors: E D Lewis; S Chen; A Kumar; G Blanck; R E Pollack; J L Manley
Journal: Proc Natl Acad Sci U S A Date: 1983-12 Impact factor: 11.205