Literature DB >> 6266915

Isolation of beta-globin-related genes from a human cosmid library.

F G Grosveld, H H Dahl, E de Boer, R A Flavell.   

Abstract

A human gene library was constructed using an improved cloning technique for cosmid vectors. Human placental DNA was partially digested with restriction endonuclease MboI; size-fractionated and ligated to BamHI-cut and phosphatase-treated cosmid vector pJB8. After packaging in lambda phage particles, the recombinant DNA was transduced into Escherichia coli 1400 or HB101 followed by selection on ampicillin for recombinant E. coli. 150 000 recombinant-DNA-containing colonies were screened for the presence of the human beta-globin related genes. Five recombinants were isolated containing the human beta-globin locus and encompassing approx. 70 kb of human DNA.

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Year:  1981        PMID： 6266915     DOI： 10.1016/0378-1119(81)90028-7

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  118 in total

1.  Analysis of junction sequences resulting from integration at nonhomologous loci in Neurospora crassa.

Authors:  D K Asch; G Frederick; J A Kinsey; D D Perkins
Journal:  Genetics       Date:  1992-04       Impact factor: 4.562

2.  Extent and high frequency of a short conversion between the human A gamma and G gamma fetal globin genes.

Authors:  J Starck; R Bouhass; F Morlé; J Godet
Journal:  Hum Genet       Date:  1990-01       Impact factor: 4.132

3.  Genetic and molecular analysis of the proximal region of the mouse t-complex using new molecular probes and partial t-haplotypes.

Authors:  C A Howard; G R Gummere; M F Lyon; D Bennett; K Artzt
Journal:  Genetics       Date:  1990-12       Impact factor: 4.562

4.  Molecular cloning and characterization of the chloroplast ribosomal RNA genes from Spirodela oligorhiza.

Authors:  R J Keus; D J Roovers; H van Heerikhuizen; G S Groot
Journal:  Curr Genet       Date:  1983-03       Impact factor: 3.886

5.  Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence.

Authors:  R Schuh; D Vestweber; I Riede; M Ringwald; U B Rosenberg; H Jäckle; R Kemler
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

6.  Globoside-specific adhesins of uropathogenic Escherichia coli are encoded by similar trans-complementable gene clusters.

Authors:  B Lund; F P Lindberg; M Båga; S Normark
Journal:  J Bacteriol       Date:  1985-06       Impact factor: 3.490

7.  Identification and mapping of origins of DNA replication within the DNA sequences of the genome of insect iridescent virus type 6.

Authors:  M Handermann; P Schnitzler; A Rösen-Wolff; K Raab; K C Sonntag; G Darai
Journal:  Virus Genes       Date:  1992-01       Impact factor: 2.332

8.  GT to AT transition at a splice donor site causes skipping of the preceding exon in phenylketonuria.

Authors:  J Marvit; A G DiLella; K Brayton; F D Ledley; K J Robson; S L Woo
Journal:  Nucleic Acids Res       Date:  1987-07-24       Impact factor: 16.971

9.  Structural organization of the 3' half of the rat thyroglobulin gene.

Authors:  V E Avvedimento; A M Musti; S Obici; S Cocozza; R Di Lauro
Journal:  Nucleic Acids Res       Date:  1984-04-25       Impact factor: 16.971

10.  Molecular cloning of the mouse ouabain-resistance gene.

Authors:  R Levenson; V Racaniello; L Albritton; D Housman
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205
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