Literature DB >> 24173112

Molecular cloning and characterization of the chloroplast ribosomal RNA genes from Spirodela oligorhiza.

R J Keus1, D J Roovers, H van Heerikhuizen, G S Groot.   

Abstract

The organization of the chloroplast ribosomal RNA genes in Spirodela oligorhiza has been determined. We have therefore characterized two cloned BamHI fragments containing the genes for the large and small rRNA genes, and two PstI fragments, each containing one of the two large, invertedly repeated regions of chloroplast DNA. Characterization was performed by restriction endonuclease mapping, DNA/RNA hybridization and electronmicroscopic R-loop analysis.The results show that the rRNA genes are present in the order 16S, 23S, 4.5S and 5S. The 16S (1.5 kb) and 23S (2.9 kb) rRNA genes are separated by a spacer of 2.25 kb. There appears to be a close resemblance between the organization of the chloroplast rRNA genes in Spirodela oligorhiza and other higher plants so far examined.

Entities:  

Year:  1983        PMID： 24173112     DOI： 10.1007/BF00365674

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  22 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Physical mapping of 4S RNA genes on chloroplast DNA of Spirodela oligorhiza.

Authors:  G S Groot; N van Harten-Loosbroek
Journal:  Curr Genet       Date:  1981-12       Impact factor: 3.886

3.  Electron microscopical localisation of the 23S and 16S rRNA genes within an inverted repeat for two chromoplast DNAs.

Authors:  J A Thompson; P Hansmann; R Knoth; G Link; H Falk
Journal:  Curr Genet       Date:  1981-09       Impact factor: 3.886

4.  Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors:  M Dagert; S D Ehrlich
Journal:  Gene       Date:  1979-05       Impact factor: 3.688

5.  Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes.

Authors:  W Koch; K Edwards; H Kössel
Journal:  Cell       Date:  1981-07       Impact factor: 41.582

6.  The nucleotide sequence of the intergenic region between the 5.8S and 26S rRNA genes of the yeast ribosomal RNA operon. Possible implications for the interaction between 5.8S and 26S rRNA and the processing of the primary transcript.

Authors:  G M Veldman; J Klootwijk; H van Heerikhuizen; R J Planta
Journal:  Nucleic Acids Res       Date:  1981-10-10       Impact factor: 16.971

7.  Localization of 4S RNA genes on the chloroplast genome of Chlamydomonas reinhardii.

Authors:  P Malnoë; J D Rochaix
Journal:  Mol Gen Genet       Date:  1978-11-09

8.  Nucleotide sequence of wheat chloroplastid 4.5 S ribonucleic acid. Sequence homologies in 4.5 S RNA species.

Authors:  A G Wildeman; R N Nazar
Journal:  J Biol Chem       Date:  1980-12-25       Impact factor: 5.157

9.  Mapping adenines, guanines, and pyrimidines in RNA.

Authors:  H Donis-Keller; A M Maxam; W Gilbert
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

10.  Mapping of the ribosomal RNA genes on spinach chloroplast DNA.

Authors:  P R Whitfeld; R G Herrmann; W Bottomley
Journal:  Nucleic Acids Res       Date:  1978-06       Impact factor: 16.971
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  2 in total

1.  Isolation, characterization, phosphorylation and site of synthesis of Spinacia chloroplast ribosomal proteins.

Authors:  M Posno; M van Noort; R Débise; G S Groot
Journal:  Curr Genet       Date:  1984-02       Impact factor: 3.886

2.  The genes encoding chloroplast ribosomal proteins S7 and S12 are located in the inverted repeat of Spirodela oligorhiza chloroplast DNA.

Authors:  M Posno; W R Verweij; I C Dekker; P M de Waard; G S Groot
Journal:  Curr Genet       Date:  1986       Impact factor: 3.886
  2 in total

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