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Literature DB >> 2419906

Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence.

R Schuh, D Vestweber, I Riede, M Ringwald, U B Rosenberg, H Jäckle, R Kemler.

Abstract

A clone (F20) containing coding sequences for the cell adhesion molecule uvomorulin was isolated by immunological techniques from cDNA library in the expression vector lambda gt11. The beta-galactosidase-uvomorulin fusion protein was used to affinity purify anti-uvomorulin antibodies. Affinity-purified antibodies recognized uvomorulin from cell lysates of embryonal carcinoma cells and reacted with the cell surface of embryonal carcinoma cells. The 1.8-kilobase cDNA insert hybridized to a single 4.3-kilobase poly(A)+ RNA species found only in cells expressing uvomorulin. Part of the nontranslated 3' sequences of the cloned uvomorulin cDNA is homologous to the interspersed B1 repeat of the mouse genome.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1986 PMID： 2419906 PMCID： PMC323076 DOI： 10.1073/pnas.83.5.1364

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

32 in total

1. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

Authors: S W Kessler
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2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

3. Efficient isolation of genes by using antibody probes.

Authors: R A Young; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1983-03 Impact factor: 11.205

4. Cell lines derived from teratocarcinomas.

Authors: J F Nicolas; P Avner; J Gaillard; J L Guenet; H Jakob; F Jacob
Journal: Cancer Res Date: 1976-11 Impact factor: 12.701

5. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.

Authors: J Messing; J Vieira
Journal: Gene Date: 1982-10 Impact factor: 3.688

6. Rapid and quantitative recovery of DNA fragments from gels by displacement electrophoresis (isotachophoresis).

Authors: L G Ofverstedt; K Hammarström; N Balgobin; S Hjertén; U Pettersson; J Chattopadhyaya
Journal: Biochim Biophys Acta Date: 1984-06-16

7. Mechanisms of adhesion among cells from neural tissues of the chick embryo.

Authors: U Rutishauser; J P Thiery; R Brackenbury; B A Sela; G M Edelman
Journal: Proc Natl Acad Sci U S A Date: 1976-02 Impact factor: 11.205

8. Rabbit antiserum against a purified surface glycoprotein decompacts mouse preimplantation embryos and reacts with specific adult tissues.

Authors: D Vestweber; R Kemler
Journal: Exp Cell Res Date: 1984-05 Impact factor: 3.905

9. Alkaline phosphatase activity in mouse teratoma.

Authors: E G Berstine; M L Hooper; S Grandchamp; B Ephrussi
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205

10. A cell surface glycoprotein involved in the compaction of embryonal carcinoma cells and cleavage stage embryos.

Authors: F Hyafil; D Morello; C Babinet; F Jacob
Journal: Cell Date: 1980-10 Impact factor: 41.582

14 in total

1. Cadherins in maternal-foetal interactions: red queen with a green beard?

Authors: Kyle Summers; Bernard Crespi
Journal: Proc Biol Sci Date: 2005-03-22 Impact factor: 5.349

Review 2. Discovering the molecular components of intercellular junctions--a historical view.

Authors: Werner W Franke
Journal: Cold Spring Harb Perspect Biol Date: 2009-09 Impact factor: 10.005

Review 3. Epithelial cell adhesion mechanisms.

Authors: B Boyer; J P Thiery
Journal: J Membr Biol Date: 1989-12 Impact factor: 1.843

Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence.

1. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

3. Efficient isolation of genes by using antibody probes.

4. Cell lines derived from teratocarcinomas.

5. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.

6. Rapid and quantitative recovery of DNA fragments from gels by displacement electrophoresis (isotachophoresis).

7. Mechanisms of adhesion among cells from neural tissues of the chick embryo.

8. Rabbit antiserum against a purified surface glycoprotein decompacts mouse preimplantation embryos and reacts with specific adult tissues.

9. Alkaline phosphatase activity in mouse teratoma.

10. A cell surface glycoprotein involved in the compaction of embryonal carcinoma cells and cleavage stage embryos.

1. Cadherins in maternal-foetal interactions: red queen with a green beard?

Review 2. Discovering the molecular components of intercellular junctions--a historical view.

Review 3. Epithelial cell adhesion mechanisms.

Review 4. Molecular biology of cadherins in the nervous system.

5. Expression of c-ets-1 mRNA is associated with an invasive, EMT-derived phenotype in breast carcinoma cell lines.

Review 6. Should I stay or should I go? Cadherin function and regulation in the neural crest.

7. Sequence analysis of a cDNA clone encoding the liver cell adhesion molecule, L-CAM.

Review 8. To adhere or not to adhere: the role of Cadherins in neural crest development.

9. Expression of the cell-cell adhesion glycoprotein cell-CAM 120/80 in normal human tissues and tumors.

10. Chromosomal mapping of the structural gene coding for the mouse cell adhesion molecule uvomorulin.