Literature DB >> 6265875

Synthesis of a model promoter for gene expression in Escherichia coli.

V N Dobrynin, V G Korobko, I V Severtsova, N S Bystrov, S A Chuvpilo, M N Kolosov.   

Abstract

We have synthesized a 67-membered double-stranded DNA containing putative initiation sites for transcription and translation in E. coli. Among the potential applications of this DNA are construction of artificial genes, cloning of foreign genes in E. coli, and study of structure - activity relations in promoter and ribosome binding sites.

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Year:  1980        PMID： 6265875

Source DB:  PubMed          Journal:  Nucleic Acids Symp Ser        ISSN： 0261-3166


  11 in total

1.  A novel method for promoter search enhanced by function-specific subgrouping of promoters--developed and tested on E.coli system.

Authors:  F Rozkot; P Sázelová; L Pivec
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971

2.  Promoters recognized by Escherichia coli RNA polymerase selected by function: highly efficient promoters from bacteriophage T5.

Authors:  R Gentz; H Bujard
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

3.  Quantitative sequence-activity models (QSAM)--tools for sequence design.

Authors:  J Jonsson; T Norberg; L Carlsson; C Gustafsson; S Wold
Journal:  Nucleic Acids Res       Date:  1993-02-11       Impact factor: 16.971

4.  Gene expression: chemical synthesis and molecular cloning of a bacteriophage T5 (T5P25) early promoter.

Authors:  J Rommens; D MacKnight; L Pomeroy-Cloney; E Jay
Journal:  Nucleic Acids Res       Date:  1983-09-10       Impact factor: 16.971

5.  Biological expression of an Escherichia coli consensus sequence promoter and some mutant derivatives.

Authors:  J J Rossi; X Soberon; Y Marumoto; J McMahon; K Itakura
Journal:  Proc Natl Acad Sci U S A       Date:  1983-06       Impact factor: 11.205

6.  Solid-phase methods for sequencing of nucleic acids I. Simultaneous sequencing of different oligodeoxyribonucleotides using a new, mechanically stable anion-exchange paper.

Authors:  A Rosenthal; S Schwertner; V Hahn; H D Hunger
Journal:  Nucleic Acids Res       Date:  1985-02-25       Impact factor: 16.971

7.  Mouse ubiquitous B2 repeat in polysomal and cytoplasmic poly(A)+RNAs: uniderectional orientation and 3'-end localization.

Authors:  A P Ryskov; P L Ivanov; D A Kramerov; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1983-09-24       Impact factor: 16.971

8.  The construction of a synthetic Escherichia coli trp promoter and its use in the expression of a synthetic interferon gene.

Authors:  J D Windass; C R Newton; J De Maeyer-Guignard; V E Moore; A F Markham; M D Edge
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

9.  Chemical synthesis and biochemical reactivity of bacteriophage lambda PR promoter.

Authors:  P L deHaseth; R A Goldman; C L Cech; M H Caruthers
Journal:  Nucleic Acids Res       Date:  1983-02-11       Impact factor: 16.971

10.  Functional dissection of Escherichia coli promoters: information in the transcribed region is involved in late steps of the overall process.

Authors:  W Kammerer; U Deuschle; R Gentz; H Bujard
Journal:  EMBO J       Date:  1986-11       Impact factor: 11.598
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