Literature DB >> 6265504

Enzyme-linked immunosorbent assay for measurement of antibody against cytomegalovirus and rubella virus in a single serum dilution.

A M van Loon, J T van der Logt, J van der Veen.   

Abstract

Enzyme-linked immunosorbent assays (ELISA) were developed for quantifying cytomegalovirus (CMV) and rubella antibodies using a single serum dilution (1/800) in conjunction with a standard curve. A near linear relation was found between the logarithms of absorbance values of sera at a dilution of 1/800 and the titres as determined by an end point dilution ELISA. The reproducibility of the single dilution ELISA was good; the within-test coefficients of variation averaged 7.5% for CMV antibody and 12.4% for rubella antibody. A close correlation was found between ELISA and complement-fixing (CF) antibody titres to CMV and between ELISA and haemagglutination-inhibition (HI) antibody titres to rubella virus. The titres in ELISA were 200 to 1000 times higher than in CF for CMV and 50 to 100 times higher than in HI for rubella virus.

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Year:  1981        PMID： 6265504      PMCID： PMC493646          DOI： 10.1136/jcp.34.6.665

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  23 in total

1.  Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus.

Authors:  H Schmitz; H W Doerr; D Kampa; A Vogt
Journal:  J Clin Microbiol       Date:  1977-06       Impact factor: 5.948

2.  Use of isolated nuclei in the indirect fluorescent-antibody test for human cytomegalovirus infection: comparison with microneutralization, anticomplement, and conventional indirect fluorescent-antibody assays.

Authors:  S Stagno; D W Reynolds; R J Smith
Journal:  J Clin Microbiol       Date:  1978-05       Impact factor: 5.948

3.  Improved glycine-extracted complement-fixing antigen for human cytomegalovirus.

Authors:  J D Kettering; N J Schmidt; E H Lennette
Journal:  J Clin Microbiol       Date:  1977-12       Impact factor: 5.948

4.  Enzyme-linked immunosorbent assay determination of specific rubella antibody levels in micrograms of immunoglobulin G per milliliter of serum in clinical samples.

Authors:  P O Leinikki; I Shekarchi; P Dorsett; J L Sever
Journal:  J Clin Microbiol       Date:  1978-10       Impact factor: 5.948

5.  Growth of high titered rubella virus in roller bottle cultures of Vero cells.

Authors:  H Liebhaber; T Pajot; J T Riordan
Journal:  Proc Soc Exp Biol Med       Date:  1969-01

6.  Experience with a modified rubella hemagglutination inhibition antibody test.

Authors:  L Z Cooper; B Matters; J K Rosenblum; S Krugman
Journal:  JAMA       Date:  1969-01-06       Impact factor: 56.272

7.  Detection of antibody to rubella virus by enzyme-linked immunosorbent assay.

Authors:  M Gravell; P H Dorsett; O Gutenson; A C Ley
Journal:  J Infect Dis       Date:  1977-10       Impact factor: 5.226

Review 8.  Enzyme immunoassays with special reference to ELISA techniques.

Authors:  A Voller; A Bartlett; D E Bidwell
Journal:  J Clin Pathol       Date:  1978-06       Impact factor: 3.411

9.  Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination.

Authors:  A Voller; D E Bidwell
Journal:  Br J Exp Pathol       Date:  1976-04

10.  Rubella-virus hemagglutination-inhibition test.

Authors:  G L Stewart; P D Parkman; H E Hopps; R D Douglas; J P Hamilton; H M Meyer
Journal:  N Engl J Med       Date:  1967-03-09       Impact factor: 91.245
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  14 in total

1.  Comparison of four techniques for detection of antibodies to cytomegalovirus.

Authors:  Y J Kraat; R M Hendrix; M P Landini; C A Bruggeman
Journal:  J Clin Microbiol       Date:  1992-02       Impact factor: 5.948

2.  Detection of immunoglobulin M and G antibodies against cytomegalovirus early and late antigens by enzyme-linked immunosorbent assay.

Authors:  J M Middeldorp; J Jongsma; A ter Haar; J Schirm; T H The
Journal:  J Clin Microbiol       Date:  1984-10       Impact factor: 5.948

3.  Immunoenzymatic assay of anti-diphtheric toxin antibodies in human serum.

Authors:  M E Camargo; L Silveira; J A Furuta; E P Oliveira; O A Germek
Journal:  J Clin Microbiol       Date:  1984-10       Impact factor: 5.948

4.  Large-scale serological screening for cytomegalovirus antibodies in homosexual males by enzyme-linked immunosorbent assay.

Authors:  J S Dylewski; L Rasmussen; J Mills; T C Merigan
Journal:  J Clin Microbiol       Date:  1984-02       Impact factor: 5.948

5.  Viral antibody screening system that uses a standardized single dilution immunoglobulin G enzyme immunoassay with multiple antigens.

Authors:  J Blomberg; I Nilsson; M Andersson
Journal:  J Clin Microbiol       Date:  1983-06       Impact factor: 5.948

6.  Normalized enzyme-linked immunosorbent assay for determining immunoglobulin G antibodies to cytomegalovirus.

Authors:  D J Kiefer; D A Phelps; S P Halbert
Journal:  J Clin Microbiol       Date:  1983-07       Impact factor: 5.948

7.  Detection of immunoglobulin G antibodies to cytomegalovirus antigens by antibody capture enzyme-linked immunosorbent assay.

Authors:  S L Nielsen; E Rønholm; I Sørensen; H K Andersen
Journal:  J Clin Microbiol       Date:  1986-12       Impact factor: 5.948

Review 8.  Congenital cytomegalovirus infection after recurrent infection: case reports and review of the literature.

Authors:  Michael A Gaytant; G Ingrid J G Rours; Eric A P Steegers; Jochem M D Galama; Ben A Semmekrot
Journal:  Eur J Pediatr       Date:  2003-02-13       Impact factor: 3.183

9.  Comparison of enzyme-linked immunosorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemagglutination techniques for detecting cytomegalovirus IgG antibody.

Authors:  J C Booth; G Hannington; T M Bakir; H Stern; H Kangro; P D Griffiths; R B Heath
Journal:  J Clin Pathol       Date:  1982-12       Impact factor: 3.411

10.  New latex agglutination test for rapid determination of rubella immune status.

Authors:  A S Weissfeld; A C Sonnenwirth
Journal:  J Clin Microbiol       Date:  1982-11       Impact factor: 5.948
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