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Literature DB >> 6255438

A comparison of DNA cleavage by the restriction enzymes SalPI and PstI.

J A Carter, K F Chater, C J Bruton, N L Brown.

Abstract

Methods for obtaining highly active, exonuclease-free, stable preparations of the Streptomyces albus P restriction enzyme SalPI are described. SalPI and its isoschizomer PstI (from the taxonomically distant Providencia stuartii 164) both cleave their recognition sequence (5'-CTGCAG-3') to generate fragments terminating in tetranucleotide 3' extensions whose sequence is 5'-TGCA-3'. Bacteriophage R4G2 DNA, protected against SalPI cleavage by pregrowth on S. albus P, is also protected against PstI cleavage; and total DNA of both S. albus P and P. stuartii 164 is resistant to cleavage by both enzymes.

Entities: Chemical Species

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Year: 1980 PMID： 6255438 PMCID： PMC324271 DOI： 10.1093/nar/8.21.4943

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

22 in total

1. A site-specific endodeoxyribonuclease from Streptomyces albus CMI 52766 sharing site-specificity with Providencia stuartii endonuclease PstI.

Authors: K F Chater
Journal: Nucleic Acids Res Date: 1977-06 Impact factor: 16.971

2. The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I.

Authors: N L Brown; M Smith
Journal: FEBS Lett Date: 1976-06-15 Impact factor: 4.124

3. A simple, general procedure for purifying restriction endonucleases.

Authors: T A Bickle; V Pirrotta; R Imber
Journal: Nucleic Acids Res Date: 1977-08 Impact factor: 16.971

4. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.

Authors: G N Godson; D Vapnek
Journal: Biochim Biophys Acta Date: 1973-04-11

5. Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA.

Authors: F Galibert; J Sedat; E Ziff
Journal: J Mol Biol Date: 1974-08-15 Impact factor: 5.469

6. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

Authors: R R Burgess
Journal: J Biol Chem Date: 1969-11-25 Impact factor: 5.157

7. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.

Authors: D I Smith; F R Blattner; J Davies
Journal: Nucleic Acids Res Date: 1976-02 Impact factor: 16.971

8. Restriction of a bacteriophage of Streptomyces albus G involving endonuclease SalI.

Authors: K F Chater; L C Wilde
Journal: J Bacteriol Date: 1976-11 Impact factor: 3.490

9. Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria.

Authors: R Benveniste; J Davies
Journal: Proc Natl Acad Sci U S A Date: 1973-08 Impact factor: 11.205

9. The glycerol utilization operon of Streptomyces coelicolor: genetic mapping of gyl mutations and the analysis of cloned gylDNA.

Authors: E T Seno; C J Bruton; K F Chater
Journal: Mol Gen Genet Date: 1984

10. Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans.

Authors: M J Bibb; J M Ward; T Kieser; S N Cohen; D A Hopwood
Journal: Mol Gen Genet Date: 1981

10 in total

A comparison of DNA cleavage by the restriction enzymes SalPI and PstI.

1. A site-specific endodeoxyribonuclease from Streptomyces albus CMI 52766 sharing site-specificity with Providencia stuartii endonuclease PstI.

2. The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I.

3. A simple, general procedure for purifying restriction endonucleases.

4. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.

5. Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA.

6. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

7. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.

8. Restriction of a bacteriophage of Streptomyces albus G involving endonuclease SalI.

9. Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria.

10. DNA sequencing with chain-terminating inhibitors.

1. Restriction enzymes and their isoschizomers.

2. Restriction enzymes and their isoschizomers.

3. Restriction and modification enzymes and their recognition sequences.

4. Restriction enzymes and their isoschizomers.

5. The effect of sequence specific DNA methylation on restriction endonuclease cleavage.

6. Restriction and modification enzymes and their recognition sequences.

7. The effect of site specific methylation on restriction endonuclease cleavage (update).

8. The chloroplast DNAs of flax genotrophs.

9. The glycerol utilization operon of Streptomyces coelicolor: genetic mapping of gyl mutations and the analysis of cloned gylDNA.

10. Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans.