Literature DB >> 6273824

The effect of several nucleic acid binding drugs on the cleavage of d(GGAATTCC) and pBR 322 by the Eco RI restriction endonuclease.

M Goppelt, J Langowski, A Pingoud, W Haupt, C Urbanke, H Mayer, G Maass.   

Abstract

The endonucleolytic action of the EcoRI restriction enzyme on the double-stranded oligonucleotide d(GGAATTCC) and the supercoiled plasmid DNA pBR 233 is inhibited by actinomycin D, ethidium bromide, proflavin, distamycin A and netropsin. Half-maximal inhibition is observed at around 100 microM concentrations for the intercalating drugs, and around 0.1 to 1 microM concentrations for netropsin and distamycin A. The inhibitory activity of these drugs can be correlated with their affinity to the oligonucleotide and the plasmid DNA. Since at high concentrations of the drugs a complete inhibition is observed, it is concluded that the effect of the drugs on the stereochemistry of the EcoRI site is such that recognition is excluded.

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Year:  1981        PMID： 6273824      PMCID： PMC327588          DOI： 10.1093/nar/9.22.6115

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

Review 1.  Effects of the antibiotics netropsin and distamycin A on the structure and function of nucleic acids.

Authors:  C Zimmer
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1975

Review 2.  Structures and mechanisms of DNA restriction and modification enzymes.

Authors:  P Modrich
Journal:  Q Rev Biophys       Date:  1979-08       Impact factor: 5.318

3.  The use of ethidium bromide-circular DNA complexes for the fluorometric analysis of breakage and joining of DNA.

Authors:  C Paoletti; J B LePecq; I R Lehman
Journal:  J Mol Biol       Date:  1971-01-14       Impact factor: 5.469

4.  Complex formation between ethidium bromide and nucleic acids.

Authors:  M J Waring
Journal:  J Mol Biol       Date:  1965-08       Impact factor: 5.469

5.  Numerical analysis of binding studies: a direct procedure avoiding the pitfalls of a Scatchard analysis of equilibrium data for unknown binding models.

Authors:  F Peters; A Pingoud
Journal:  Int J Biomed Comput       Date:  1979-09

6.  Transient cleavage kinetics of the Eco RI restriction endonuclease measured in a pulsed quench-flow apparatus: enzyme concentration-dependent activity change.

Authors:  J Langowski; C Urbanke; A Pingoud; G Maass
Journal:  Nucleic Acids Res       Date:  1981-07-24       Impact factor: 16.971

Review 7.  Restriction and modification enzymes and their recognition sequences.

Authors:  R J Roberts
Journal:  Nucleic Acids Res       Date:  1980-01-11       Impact factor: 16.971

8.  The interaction of the EcoRI restriction endonuclease with its substrate. A physico-chemical study employing natural and synthetic oligonucleotides and polynucleotides.

Authors:  M Goppelt; A Pingoud; G Maass; H Mayer; H Köster; R Frank
Journal:  Eur J Biochem       Date:  1980-02

9.  Protection of particular cleavage sites of restriction endonucleases by distamycin A and actinomycin D.

Authors:  V V Nosikov; E A Braga; A V Karlishev; A L Zhuze; O L Polyanovsky
Journal:  Nucleic Acids Res       Date:  1976-09       Impact factor: 16.971

10.  Specific interactions of distamycin A and its analogs with (A-T) rich and (G-C) rich duplex regions of DNA and deoxypolynucleotides.

Authors:  G Luck; C Zimmer; K E Reinert; F Arcamone
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971
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  6 in total

1.  The selective inhibitory effect of netropsin on relaxation of sequence specificity of restriction endonuclease SgrAI recognizing the octanucleotide sequence 5'-CR decreases CCGGYG-3'.

Authors:  F Laue; W Ankenbauer; G G Schmitz; C Kessler
Journal:  Nucleic Acids Res       Date:  1990-06-11       Impact factor: 16.971

2.  'Star' activity and complete loss of specificity of CeqI endonuclease.

Authors:  Z Izsvák; E Duda
Journal:  Biochem J       Date:  1989-02-15       Impact factor: 3.857

3.  Alkyl phosphotriester modified oligodeoxyribonucleotides. V. Synthesis and absolute configuration of Rp and Sp diastereomers of an ethyl phosphotriester (Et) modified EcoRI recognition sequence, d[GGAA(Et)TTCC]. A synthetic approach to regio- and stereospecific ethylation-interference studies.

Authors:  K A Gallo; K L Shao; L R Phillips; J B Regan; M Koziolkiewicz; B Uznanski; W J Stec; G Zon
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

4.  Aggregation of DNA by analogs of spermidine; enzymatic and structural studies.

Authors:  K S Srivenugopal; D E Wemmer; D R Morris
Journal:  Nucleic Acids Res       Date:  1987-03-25       Impact factor: 16.971

5.  Phosphorothioate-modified oligodeoxyribonucleotides. III. NMR and UV spectroscopic studies of the Rp-Rp, Sp-Sp, and Rp-Sp duplexes, [d(GGSAATTCC)]2, derived from diastereomeric O-ethyl phosphorothioates.

Authors:  L A LaPlanche; T L James; C Powell; W D Wilson; B Uznanski; W J Stec; M F Summers; G Zon
Journal:  Nucleic Acids Res       Date:  1986-11-25       Impact factor: 16.971

6.  Alleviation of restriction by DNA condensation and non-specific DNA binding ligands.

Authors:  Steven A Keatch; Tsueu-Ju Su; David T F Dryden
Journal:  Nucleic Acids Res       Date:  2004-11-01       Impact factor: 16.971
  6 in total

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