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Literature DB >> 6253889

Alterations in two conserved regions of promoter sequence lead to altered rates of polymerase binding and levels of gene expression.

Abstract

Characterization of recombinant lac promoters highlights the importance of two regions of sequence conservation in promoters. The "Pribnow box" sequences are necessary for specific transcription in this system. This specificity is maintained when a mutated upstream sequence is introduced. However, changing the upstream DNA sequences influences both the rate of RNA polymerase binding in vitro and levels of expression in vivo.

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Year: 1980 PMID： 6253889 PMCID： PMC324115 DOI： 10.1093/nar/8.12.2709

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

27 in total

1. Genetic regulation: the Lac control region.

Authors: R C Dickson; J Abelson; W M Barnes; W S Reznikoff
Journal: Science Date: 1975-01-10 Impact factor: 47.728

2. The location of the repressor binding sites in the lac operon.

Authors: W S Reznikoff; R B Winter; C K Hurley
Journal: Proc Natl Acad Sci U S A Date: 1974-06 Impact factor: 11.205

Review 3. The selectivity of transcription.

Authors: M J Chamberlin
Journal: Annu Rev Biochem Date: 1974 Impact factor: 23.643

4. Studies of the binding of Escherichia coli RNA polymerase to DNA. II. The kinetics of the binding reaction.

Authors: D C Hinkle; M J Chamberlin
Journal: J Mol Biol Date: 1972-09-28 Impact factor: 5.469

5. Evidence for two sites in the lac promoter region.

Authors: J Beckwith; T Grodzicker; R Arditti
Journal: J Mol Biol Date: 1972-08-14 Impact factor: 5.469

6. A new class of promoter mutations in the lactose operon of Escherichia coli.

Authors: J D Hopkins
Journal: J Mol Biol Date: 1974-08-25 Impact factor: 5.469

7. Regulation of lac mRNA synthesis in a soluble cell-free system.

Authors: B De Crombrugghe; B Chen; M Gottesman; I Pastan; H E Varmus; M Emmer; R L Perlman
Journal: Nat New Biol Date: 1971-03-10

8. The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli.

Authors: N M Maizels
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205

8. DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus.

Authors: P Dierks; A van Ooyen; N Mantei; C Weissmann
Journal: Proc Natl Acad Sci U S A Date: 1981-03 Impact factor: 11.205

9. The context of gene expression regulation.

Authors: Johan H Gibcus; Job Dekker
Journal: F1000 Biol Rep Date: 2012-04-02

10. Transcription initiation by mix and match elements: flexibility for polymerase binding to bacterial promoters.

Authors: India G Hook-Barnard; Deborah M Hinton
Journal: Gene Regul Syst Bio Date: 2007

10 in total

Alterations in two conserved regions of promoter sequence lead to altered rates of polymerase binding and levels of gene expression.

1. Genetic regulation: the Lac control region.

2. The location of the repressor binding sites in the lac operon.

Review 3. The selectivity of transcription.

4. Studies of the binding of Escherichia coli RNA polymerase to DNA. II. The kinetics of the binding reaction.

5. Evidence for two sites in the lac promoter region.

6. A new class of promoter mutations in the lactose operon of Escherichia coli.

7. Regulation of lac mRNA synthesis in a soluble cell-free system.

8. The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli.

9. Catabolite-insensitive revertants of lac promoter mutants.

10. Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd.

1. Escherichia coli promoter opening and -10 recognition: mutational analysis of sigma70.

2. Dual mechanism of repression at a distance in the lac operon.

3. The interaction of RNA polymerase II with non-promoter DNA sites.

4. Characterization of the beta-lactamase promoter of pBR322.

5. Spacer mutations in the lac ps promoter.

6. Segment-specific mutagenesis: extensive mutagenesis of a lac promoter/operator element.

7. The mac promoters: functional hybrid promoters activated by the malT product and repressed by the lacI product.

8. DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus.

9. The context of gene expression regulation.

10. Transcription initiation by mix and match elements: flexibility for polymerase binding to bacterial promoters.