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Literature DB >> 6269053

Characterization of the beta-lactamase promoter of pBR322.

Abstract

The beta-lactamase promoter of pBR322, derived from Tn3, has been characterized using several techniques. The transcription initiation site is located 35 base pairs from the translation initiation codon of beta-lactamase. The mRNA produced in vitro has a 5' pppGpA terminus. RNA polymerase bound at this start site protects a region from about -50 to +20 from DNase I cleavage using the footprinting technique. RNA polymerase binds rapidly to the beta-lactamase promoter. The half-time of association is less than one-half minute. The half-time of dissociation is approximately 6 hr. A study of the binding of RNA polymerase at different temperatures showed a large change between 11 degrees and 15 degrees C. Comparison of these parameters with those reported for other promoters is discussed.

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Year: 1981 PMID： 6269053 PMCID： PMC326868 DOI： 10.1093/nar/9.11.2517

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

35 in total

1. Sequence determination of 5'-terminal and 3'-terminal T1 oligonucleotides of 18-S ribosomal RNA of a mouse cell line (L 5178 Y).

Authors: M E Eladari; F Galibert
Journal: Eur J Biochem Date: 1975-06-16

2. Interaction of RNA polymerase with promoters from bacteriophage fd.

Authors: P H Seeburg; C Nüsslein; H Schaller
Journal: Eur J Biochem Date: 1977-03-15

3. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

4. Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. 3. The effect of temperature on ribonucleic acid chain initiation and on the conformation of binary complexes.

Authors: W F Mangel; M J Chamberlin
Journal: J Biol Chem Date: 1974-05-25 Impact factor: 5.157

5. Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection.

Authors: D C Hinkle; M J Chamberlin
Journal: J Mol Biol Date: 1972-09-28 Impact factor: 5.469

6. Stimulation by cyclic AMP and ppGpp of chloramphenicol acetyl transferase synthesis.

Authors: B De Crombrugghe; I Pastan; W V Shaw; J L Rosner
Journal: Nat New Biol Date: 1973-02-21

7. Resistance of Escherichia coli to penicillins. V. Physiological comparison of two isogenic strains, one with chromosomally and one with episomally mediated ampicillin resistance.

Authors: L G Burman; K Nordström; H G Boman
Journal: J Bacteriol Date: 1968-08 Impact factor: 3.490

8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

9. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.

Authors: R R Burgess; J J Jendrisak
Journal: Biochemistry Date: 1975-10-21 Impact factor: 3.162

10. Nucleoside triphosphate termini from RNA synthesized in vivo by Escherichia coli.

Authors: S E Jorgensen; L B Buch; D P Nierlich
Journal: Science Date: 1969-05-30 Impact factor: 47.728

21 in total

1. Patents and literature.

Authors: A Rembaum; R P Rohrbach; S Klose; H G Batz; M Stoltz; A Hagen; G Weimann; S G Wildman; P Kwanyuen; G Franzmann; H L Wulsmann; T Nakanishi; Y Shigemasa; R Monsheimer; E Pfleiderer; T Yoshioka; K Teramoto; M Shimamura; C K Lee; M Tamuri; M Kanno; Y Ishi; I Kaetsu; M Kumakura; M Yoshida; G A Strobel; D E Johnson; V R Srinivasan; Y C Choi; H Fukuda; T Shiotani; W Okada; R A Messing; R A Oppermann; L B Simpson; M M Takeguchi; M Bigazzi; T B Higerd; J M Goust; W R Tolbert; M J Kuo; J Feder; R N Patel; C T Hou; A I Laskin; H Yukawa; K Osumi; Y Takayama; A Fujiwara; S Masuda; F Takaku; K Ogasa; M Kuboyama; M Saito; N Yanai; M Nishida; O Tosaka; E Ono; M Ishihara; H Morioka; K Takinami; A Taoka; S Uchida; J Puls; M Sinner; H H Dietrichs; D T Wicklow; R W Detroy; R E Heady; H Yukawa; K Osumi; T Nara; Y Takayama; E Knight; G T Tsao; M R Ladisch; C M Ladisch; T A Hsu; H J Friesen; S Pestka; J J Manis; V G Debabov; J I Kozlov; N I Zhdanova; E M Khurges; N K Yankovsky; M R Rozinov; R S Shakulov; B A Rebentish; V A Livshits; M M Gusyatiner; S V Mashko; V N Moshentseva; L F Kozyreva; R A Arsatiants; H M Goodman; J Shine; P Horst; D V Goeddel; H L Heyneker
Journal: Appl Biochem Biotechnol Date: 1981-12 Impact factor: 2.926

2. Identification of a glyphosate-resistant mutant of rice 5-enolpyruvylshikimate 3-phosphate synthase using a directed evolution strategy.

Authors: Min Zhou; Honglin Xu; Xiaoli Wei; Zhiqiang Ye; Liping Wei; Weimin Gong; Yongqin Wang; Zhen Zhu
Journal: Plant Physiol Date: 2005-12-16 Impact factor: 8.340

3. Alteration of the curved helical structure located in the upstream region of the beta-lactamase promoter of plasmid pUC19 and its effect on transcription.

Authors: T Ohyama; M Nagumo; Y Hirota; S Sakuma
Journal: Nucleic Acids Res Date: 1992-04-11 Impact factor: 16.971

4. Updated sequence information for TEM beta-lactamase genes.

Authors: S Goussard; P Courvalin
Journal: Antimicrob Agents Chemother Date: 1999-02 Impact factor: 5.191

5. Visualization and quantitative analysis of complex formation between E. coli RNA polymerase and an rRNA promoter in vitro.

Authors: R L Gourse
Journal: Nucleic Acids Res Date: 1988-10-25 Impact factor: 16.971

6. Mapping the B and D gene promoters of bacteriophage S13 by footprinting and exonuclease III analysis.

Authors: M Arquint; J H Spencer
Journal: J Virol Date: 1987-12 Impact factor: 5.103

7. Variations between the nucleotide sequences of Tn1, Tn2, and Tn3 and expression of beta-lactamase in Pseudomonas aeruginosa and Escherichia coli.

Authors: S T Chen; R C Clowes
Journal: J Bacteriol Date: 1987-02 Impact factor: 3.490