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Literature DB >> 6253887

Processing of ribosomal precursor RNAs in Physarum polycephalum.

Abstract

The processing of intermediates of Physarum rRNA has been investigated using two cloned rDNA restriction fragments as sequence specific probes. The size of the largest stable pre-rRNA detected is 11.8 Kb, as determined by denaturing gel electrophoresis. R-loop analysis revealed that this pre-rRNA fraction mainly consists of transcripts which do not contain the two insertion sequences present in the 26 S gene. However, a small number of molecules are found which still contain one or the other, or both, of these introns. These observations suggest that the two introns are transcribed but spliced out in a random order before RNA synthesis has reached the 3-end of the primary transcript. Transcription starts at about 17.7 Kb and stops at 4.4 Kb as measured from the ends of the linear palindromic rDNA molecule. Besides the 11.8 Kb transcript several further ribosomal RNA precursors have been identified, ranging in size from 2.5 to 8.8 Kb. In addition, the genes for 5.8 S RNA have been located by R-loop mapping.

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Year: 1980 PMID： 6253887 PMCID： PMC324111 DOI： 10.1093/nar/8.12.2647

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

39 in total

1. Secondary structure maps of ribosomal RNA and DNA. I. Processing of Xenopus laevis ribosomal RNA and structure of single-stranded ribosomal DNA.

Authors: P K Wellauer; I B Dawid
Journal: J Mol Biol Date: 1974-10-25 Impact factor: 5.469

2. Secondary structure maps of ribosomal RNA. II. Processing of mouse L-cell ribosomal RNA and variations in the processing pathway.

Authors: P K Wellauer; I B Dawid; D E Kelley; R P Perry
Journal: J Mol Biol Date: 1974-10-25 Impact factor: 5.469

3. Secondary structure maps of RNA: processing of HeLa ribosomal RNA.

Authors: P K Wellauer; I B Dawid
Journal: Proc Natl Acad Sci U S A Date: 1973-10 Impact factor: 11.205

4. A characterization of ribonucleic acid in the myxomycete Physarum polycephalum.

Authors: P W Melera; H P Rusch
Journal: Exp Cell Res Date: 1973-11 Impact factor: 3.905

5. Isolation of ribosomal RNA precursors from Physarum polycephalum.

Authors: D N Jacobson; C E Holt
Journal: Arch Biochem Biophys Date: 1973-11 Impact factor: 4.013

6. RNA of Physarum. II. Template replication and transcription in the mitotic cycle.

Authors: A Zellweger; R Braun
Journal: Exp Cell Res Date: 1971-04 Impact factor: 3.905

7. Evolution of the transcription unit of ribosomal RNA.

Authors: R P Perry; T Y Cheng; J J Freed; J R Greenberg; D E Kelley; K D Tartof
Journal: Proc Natl Acad Sci U S A Date: 1970-03 Impact factor: 11.205

8. Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolution.

Authors: U Schibler; T Wyler; O Hagenbüchle
Journal: J Mol Biol Date: 1975-05-25 Impact factor: 5.469

9. Size, complexity and abundance of a specific poly(A)-containing RNA of liver from male Xenopus induced to vitellogenin synthesis by estrogen.

Authors: W Wahli; T Wyler; R Weber; G U Ryffel
Journal: Eur J Biochem Date: 1976-07-15

7. Different chromatin structures in Physarum polycephalum: a special form of transcriptionally active chromatin devoid of nucleosomal particles.

Authors: U Scheer; H Zentgraf; H W Sauer
Journal: Chromosoma Date: 1981 Impact factor: 4.316

8. In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena.

Authors: M Carin; B F Jensen; K D Jentsch; J C Leer; O F Nielsen; O Westergaard
Journal: Nucleic Acids Res Date: 1980-12-11 Impact factor: 16.971

8 in total

Processing of ribosomal precursor RNAs in Physarum polycephalum.

1. Secondary structure maps of ribosomal RNA and DNA. I. Processing of Xenopus laevis ribosomal RNA and structure of single-stranded ribosomal DNA.

2. Secondary structure maps of ribosomal RNA. II. Processing of mouse L-cell ribosomal RNA and variations in the processing pathway.

3. Secondary structure maps of RNA: processing of HeLa ribosomal RNA.

4. A characterization of ribonucleic acid in the myxomycete Physarum polycephalum.

5. Isolation of ribosomal RNA precursors from Physarum polycephalum.

6. RNA of Physarum. II. Template replication and transcription in the mitotic cycle.

7. Evolution of the transcription unit of ribosomal RNA.

8. Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolution.

9. Size, complexity and abundance of a specific poly(A)-containing RNA of liver from male Xenopus induced to vitellogenin synthesis by estrogen.

10. Nucleotide sequence of the rightward operator of phage lambda.

1. Processing in the external transcribed spacer of ribosomal RNA from Physarum polycephalum.

2. Nucleotide sequence of a ribosomal RNA gene intron from slime mold Physarum polycephalum.

3. RNA splicing is required to make the messenger RNA for a variant surface antigen in trypanosomes.

4. Localization and DNA sequence around the initiation site of ribosomal RNA transcription in Physarum polycephalum.

5. Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila.

6. Sequence-specific cleavage of chromatin by staphylococcal nuclease can generate an atypical nucleosome pattern.

7. Different chromatin structures in Physarum polycephalum: a special form of transcriptionally active chromatin devoid of nucleosomal particles.

8. In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena.