Literature DB >> 7279671

Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila.

R Renkawitz-Pohl, L Matsumoto, S A Gerbi.   

Abstract

We have prepared a partial gene library of sheared DNA from the fungus fly, Sciara coprophila, by dA-T tailing and insertion into pBR322. Two ribosomal DNA clones which differ from the usual ribosomal DNA organization in this organism were studied in detail. Clone pBc 1L-1 has an intervening sequence of 1.4 kb, and clone pBc 6D-6 has an intervening sequence of 0.9 kb. These intervening sequences occur in about the same position in 28S rDNA, but do not appear to share sequence homology with one another. Previously we found that 90% of Sciara ribosomal DNA is homogenous and lacks an intervening sequence, and our present data explains the size heterogeneity found in most of the remaining 10%. We have found no evidence of size heterogeneity in the nontranscribed spacer.

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Year:  1981        PMID： 7279671      PMCID： PMC327389          DOI： 10.1093/nar/9.15.3747

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  56 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Further studies on the ribosomal RNA cistrons of Sciara coprophila (Diptera).

Authors:  S A Gerbi; H V Crouse
Journal:  Genetics       Date:  1976-05       Impact factor: 4.562

3.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

4.  Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.

Authors:  R B Helling; H M Goodman; H W Boyer
Journal:  J Virol       Date:  1974-11       Impact factor: 5.103

5.  Calcium-dependent bacteriophage DNA infection.

Authors:  M Mandel; A Higa
Journal:  J Mol Biol       Date:  1970-10-14       Impact factor: 5.469

6.  Localization and characterization of the ribosomal RNA cistrons in Sciara coprophila.

Authors:  S A Gerbi
Journal:  J Mol Biol       Date:  1971-06-14       Impact factor: 5.469

7.  Hybridization of RNA to double-stranded DNA: formation of R-loops.

Authors:  M Thomas; R L White; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205

8.  A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.

Authors:  P C Wensink; D J Finnegan; J E Donelson; D S Hogness
Journal:  Cell       Date:  1974-12       Impact factor: 41.582

9.  Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.

Authors:  R Roychoudhury; E Jay; R Wu
Journal:  Nucleic Acids Res       Date:  1976-04       Impact factor: 16.971

10.  Nucleotide sequence of the rightward operator of phage lambda.

Authors:  T Maniatis; A Jeffrey; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205
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  10 in total

1.  rDNA in Locusta migratoria is very variable: two introns and extensive restriction site polymorphisms in the spacer.

Authors:  M Schäfer; W Kunz
Journal:  Nucleic Acids Res       Date:  1985-02-25       Impact factor: 16.971

2.  rRNA processing: removal of only nineteen bases at the gap between 28S alpha and 28S beta rRNAs in Sciara coprophila.

Authors:  V C Ware; R Renkawitz; S A Gerbi
Journal:  Nucleic Acids Res       Date:  1985-05-24       Impact factor: 16.971

3.  Duplicated rDNA sequences of variable lengths flanking the short type I insertions in the rDNA of Drosophila melanogaster.

Authors:  H Roiha; D M Glover
Journal:  Nucleic Acids Res       Date:  1981-11-11       Impact factor: 16.971

4.  Structural organization of the two main rDNA size classes of Ascaris lumbricoides.

Authors:  E Back; F Müller; H Tobler
Journal:  Nucleic Acids Res       Date:  1984-02-10       Impact factor: 16.971

5.  Cloning and analysis of ribosomal DNA of Chironomus thummi piger and Chironomus thummi thummi. The nontranscribed spacer of Ch. th. thummi contains a highly repetitive DNA sequence.

Authors:  E R Schmidt; E A Godwin; H G Keyl; N Israelewski
Journal:  Chromosoma       Date:  1982       Impact factor: 4.316

6.  Tsetse fly rDNA: an analysis of structure and sequence.

Authors:  N C Cross; G A Dover
Journal:  Nucleic Acids Res       Date:  1987-01-12       Impact factor: 16.971

7.  Molecular and cytological characterization of repetitive DNA sequences from the centromeric heterochromatin of Sciara coprophila.

Authors:  M Carmen Escribá; Patricia G Greciano; María Méndez-Lago; Beatriz de Pablos; Vladimir A Trifonov; Malcolm A Ferguson-Smith; Clara Goday; Alfredo Villasante
Journal:  Chromosoma       Date:  2011-05-02       Impact factor: 4.316

8.  The intron boundaries and flanking rRNA coding sequences of Calliphora erythrocephala rDNA.

Authors:  V L Smith; K Beckingham
Journal:  Nucleic Acids Res       Date:  1984-02-10       Impact factor: 16.971

9.  Structural constraints in expansion segments from a midge 26S rDNA.

Authors:  E Gorab; M Garcia de Lacoba; L M Botella
Journal:  J Mol Evol       Date:  1995-12       Impact factor: 2.395

10.  Ribosomal DNA genes of Bombyx mori: a minor fraction of the repeating units contain insertions.

Authors:  R Lecanidou; T H Eickbush; F C Kafatos
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971
  10 in total

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