Literature DB >> 6252542

Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA.

G Winberg, M L Hammarskjöld.   

Abstract

A new method for isolating DNA from agarose gels is described. The method involves the simultaneous transfer of all DNA-fragments from an agarose slab gel onto DEAE-cellulose paper and the elution of the individual fragments from the paper with 1 M NaCl. DNA isolated from agarose gels in this way is susceptible to cleavage with several restriction endonucleases, and can be labeled in vitro with E coli DNA-polymerase I, T4 DNA-polymerase and T4 polynucleotide kinase. We have used the method to construct restriction endonuclease maps of adenovirus type 16 DNA.

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Year:  1980        PMID： 6252542      PMCID： PMC327265          DOI： 10.1093/nar/8.2.253

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  26 in total

1.  A rapid procedure for purification of EcoRI endonuclease.

Authors:  J Sümegi; D Breedveld; P Hossenlopp; P Chambon
Journal:  Biochem Biophys Res Commun       Date:  1977-05-09       Impact factor: 3.575

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  A simple, general procedure for purifying restriction endonucleases.

Authors:  T A Bickle; V Pirrotta; R Imber
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

4.  Isolation of incomplete adenovirus 16 particles containing viral and host cell DNA.

Authors:  M L Hammarskjöld; G Winberg; E Norrby; G Wadell
Journal:  Virology       Date:  1977-10-15       Impact factor: 3.616

5.  Close vicinity of IS1 integration sites in the leader sequence of the gal operon of E. coli.

Authors:  S Kühn; H J Fritz; P Starlinger
Journal:  Mol Gen Genet       Date:  1979-01-02

6.  A rapid method for extracting DNA from agarose gels.

Authors:  M Finkelstein; T H Rownd
Journal:  Plasmid       Date:  1978-09       Impact factor: 3.466

7.  DNA restriction site mapping of adenovirus type 16 with BamI, EcoRI, HpaI and Sa/I.

Authors:  T M Varsanyi; G Winberg; G Wadell
Journal:  FEBS Lett       Date:  1977-04-15       Impact factor: 4.124

8.  Preparative and analytical purification of DNA from agarose.

Authors:  B Vogelstein; D Gillespie
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

9.  A method for the recovery of DNA from agarose gels.

Authors:  H F Tabak; R A Flavell
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

10.  The isolation of DNA from agarose gels by electrophoretic elution onto malachite green-polyacrylamide columns.

Authors:  B Koller; H Delius; H Bünemann; W Müller
Journal:  Gene       Date:  1978-11       Impact factor: 3.688
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  65 in total

1.  A set of anonymous DNA clones as markers for mouse gene mapping.

Authors:  H Le Roy; D Simon-Chazottes; X Montagutelli; J L Guénet
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

2.  Amplification, cloning, and sequencing of a nifH segment from aquatic microorganisms and natural communities.

Authors:  J D Kirshtein; H W Paerl; J Zehr
Journal:  Appl Environ Microbiol       Date:  1991-09       Impact factor: 4.792

3.  Wound-inducible nuclear protein binds DNA fragments that regulate a proteinase inhibitor II gene from potato.

Authors:  C J Palm; M A Costa; G An; C A Ryan
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205

4.  DNA probe technology for rapid detection of Haemophilus influenzae in clinical specimens.

Authors:  F Malouin; L E Bryan; P Shewciw; J Douglas; D Li; H Van den Elzen; J R Lapointe
Journal:  J Clin Microbiol       Date:  1988-10       Impact factor: 5.948

5.  Conservative replication of double-stranded RNA in Saccharomyces cerevisiae by displacement of progeny single strands.

Authors:  R A Sclafani; W L Fangman
Journal:  Mol Cell Biol       Date:  1984-08       Impact factor: 4.272

6.  Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii.

Authors:  J P Zehr; L A McReynolds
Journal:  Appl Environ Microbiol       Date:  1989-10       Impact factor: 4.792

7.  The ail gene of Yersinia enterocolitica has a role in the ability of the organism to survive serum killing.

Authors:  D E Pierson; S Falkow
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441

8.  Construction and characterization of cDNA clones for four respiratory syncytial viral genes.

Authors:  S Venkatesan; N Elango; R M Chanock
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

9.  General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes.

Authors:  M Mackett; G L Smith; B Moss
Journal:  J Virol       Date:  1984-03       Impact factor: 5.103

10.  Isolation and characterization of genomic clones corresponding to the human type II procollagen gene.

Authors:  C M Strom; W B Upholt
Journal:  Nucleic Acids Res       Date:  1984-01-25       Impact factor: 16.971
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